Epidemiology of chlamydophila caviae-like chlamydia isolated from urethra and uterine cervix

Wataru Murao, Koichiro Wada, Akira Matsumoto, Michihisa Fujiwara, Hideto Fukushi, Toshio Kishimoto, Koichi Monden, Reiko Kariyama, Hiromi Kumon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In 2000, chlamydial strains OK133 and OK135 were isolated from 2 female patients with cervicitis. These strains were unresponsive to commercially available PCR and LCR test kits for the diagnosis of Chlamydia trachomatis infection, and their phenotypic characteristics were very similar. The OK135 nucleotide sequence in M0MP-VD2 gene closely resembled that of Chlamydophila cawae GPIC. A similar strain was isolated in 2003 from a male patient 0KM2 with urethritis, from which the strain SC10-6 was cloned by the plaque purification method. The nucleotide sequence of the entire MOMP gene of SC10-6 was exactly the same as that of OK135. Thus, the strains OK135 and SC10-6, together with OK133, have been called C. cattae-like Chlamydia. We designed primers for nested PCR assay, the product of which showed a single-band 311-bp fragment, to detect C. cattae-like Chlamydia. Of swab specimens obtained from 202 patients from 2003 to 2006 (119 male and 83 female patients), 18 specimens (8.9%) from 14 male and 4 female patients were positive, suggesting that C. cattae-like Chlamydia infection is rather common. Thus far, it has not been determined whether C. cattae-like Chlamydia is pathogenic for humans.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalActa Medica Okayama
Volume64
Issue number1
Publication statusPublished - 2010

Fingerprint

Chlamydophila
Epidemiology
Chlamydia
Urethra
Cervix Uteri
Chlamydia Infections
Nucleotides
Genes
Uterine Cervicitis
Urethritis
Polymerase Chain Reaction
Chlamydia trachomatis
Purification
Assays

Keywords

  • Chlamydophila caviae-like chlamydia
  • Epidemiology
  • Sexually transmitted infection
  • Urethra
  • Uterine cervix

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Murao, W., Wada, K., Matsumoto, A., Fujiwara, M., Fukushi, H., Kishimoto, T., ... Kumon, H. (2010). Epidemiology of chlamydophila caviae-like chlamydia isolated from urethra and uterine cervix. Acta Medica Okayama, 64(1), 1-9.

Epidemiology of chlamydophila caviae-like chlamydia isolated from urethra and uterine cervix. / Murao, Wataru; Wada, Koichiro; Matsumoto, Akira; Fujiwara, Michihisa; Fukushi, Hideto; Kishimoto, Toshio; Monden, Koichi; Kariyama, Reiko; Kumon, Hiromi.

In: Acta Medica Okayama, Vol. 64, No. 1, 2010, p. 1-9.

Research output: Contribution to journalArticle

Murao, W, Wada, K, Matsumoto, A, Fujiwara, M, Fukushi, H, Kishimoto, T, Monden, K, Kariyama, R & Kumon, H 2010, 'Epidemiology of chlamydophila caviae-like chlamydia isolated from urethra and uterine cervix', Acta Medica Okayama, vol. 64, no. 1, pp. 1-9.
Murao W, Wada K, Matsumoto A, Fujiwara M, Fukushi H, Kishimoto T et al. Epidemiology of chlamydophila caviae-like chlamydia isolated from urethra and uterine cervix. Acta Medica Okayama. 2010;64(1):1-9.
Murao, Wataru ; Wada, Koichiro ; Matsumoto, Akira ; Fujiwara, Michihisa ; Fukushi, Hideto ; Kishimoto, Toshio ; Monden, Koichi ; Kariyama, Reiko ; Kumon, Hiromi. / Epidemiology of chlamydophila caviae-like chlamydia isolated from urethra and uterine cervix. In: Acta Medica Okayama. 2010 ; Vol. 64, No. 1. pp. 1-9.
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