Epibiotic association between filamentous bacteria and the vent-associated galatheid crab, Shinkaia crosnieri (Decapoda

Anomura)

Shinji Tsuchida, Yohey Suzuki, Yoshihiro Fujiwara, Masaru Kawato, Katsuyuki Uematsu, Toshiro Yamanaka, Chitoshi Mizota, Hiroyuki Yamamoto

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The galatheid crab Shinkaia crosnieri, is the sole member of the subfamily Shinkaiinae. It is abundant and forms dense beds around active hydrothermal vents in the Okinawa Trough. Thousands of filamentous bacteria attached to the plumose setae on the ventral surface of this crab were observed using field-emission scanning electron microscopy and transmission electron microscopy. Nucleic acids were extracted from the filamentous bacteria, and the phylotypes of 16S rRNA genes were identified from 81 clones. These phylotypes were divided into three groups: Epsilonproteobacteria (74%); Gammaproteobacteria (20%); and Bacteroidetes (6%). Gamma- and major phylotypes of Epsilonproteobacteria were also detected using fluorescence in situ hybridization analysis. These Epsilon- and Gammaproteobacteria were closely related to cultured and uncultured bacteria from hydrothermal vent fields including episymbionts of vent-associated invertebrates such as Rimicaris exoculata, Alvinella pompejana, the scaly-foot snail, Kiwa hirsuta etc. The carbon isotopic compositions of the muscle of S. crosnieri and in filamentous bacteria were similar. The muscle of S. crosnieri contained monounsaturated C16 and C18 fatty acids, which are known to be characteristic of sulphur-oxidizing bacteria in H2S-rich marine habitats. Through the video images transmitted by a submersible and a remotely operated vehicle, S. crosnieri was observed to comb out its ventral setae using the third maxilliped and appeared to consume the contents. These evidences suggest the epibiotic association between S. crosnieri and the filamentous bacteria attached to the ventral setae of the crab, but the details of role and function are still unclear at the present study.

Original languageEnglish
Pages (from-to)23-32
Number of pages10
JournalJournal of the Marine Biological Association of the United Kingdom
Volume91
Issue number1
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Decapoda
crab
crabs
bacterium
setae (animal)
bacteria
epsilon-Proteobacteria
gamma-Proteobacteria
hydrothermal vent
muscle
comb (integument)
muscles
remotely operated vehicle
Ryukyu Archipelago
submersible
nucleic acid
fluorescence in situ hybridization
snail
nucleic acids
transmission electron microscopy

Keywords

  • epibiont
  • Epsilonproteobacteria
  • Gammaproteobacteria
  • Hatoma Knoll
  • hydrothermal vent
  • Okinawa Trough
  • Shinkaia crosnieri

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Epibiotic association between filamentous bacteria and the vent-associated galatheid crab, Shinkaia crosnieri (Decapoda : Anomura). / Tsuchida, Shinji; Suzuki, Yohey; Fujiwara, Yoshihiro; Kawato, Masaru; Uematsu, Katsuyuki; Yamanaka, Toshiro; Mizota, Chitoshi; Yamamoto, Hiroyuki.

In: Journal of the Marine Biological Association of the United Kingdom, Vol. 91, No. 1, 02.2011, p. 23-32.

Research output: Contribution to journalArticle

Tsuchida, Shinji ; Suzuki, Yohey ; Fujiwara, Yoshihiro ; Kawato, Masaru ; Uematsu, Katsuyuki ; Yamanaka, Toshiro ; Mizota, Chitoshi ; Yamamoto, Hiroyuki. / Epibiotic association between filamentous bacteria and the vent-associated galatheid crab, Shinkaia crosnieri (Decapoda : Anomura). In: Journal of the Marine Biological Association of the United Kingdom. 2011 ; Vol. 91, No. 1. pp. 23-32.
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