Eosinophil cationic protein shows survival effect on H9c2 cardiac myoblast cells with enhanced phosphorylation of ERK and Akt/GSK-3β under oxidative stress

Hiroko Ishii, Shigeshi Kamikawa, Satoshi Hirohata, Akifumi Mizutani, Koji Abe, Masaharu Seno, Toshitaka Oohashi, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Eosinophil cationic protein (ECP) is well known as a cationic protein contained in the basic granules of activated eosinophils. Recent studies have reported that ECP exhibits novel activities on various types of cells, including rat neonatal cardiomyocytes. Here we evaluated the effects of ECP on rat cardiac myoblast H9c2 cells. Our results showed that ECP enhanced the survival of the cells, in part by promoting the ERK and Akt/GSK-3β signaling pathways. ECP attenuated the cytotoxic effects of H2O2 on H9c2 cells as well as the production of reactive oxygen species, the number of apoptotic cells and caspase 3/7 activity in the cells. In conclusion, ECP activated the ERK and Akt/GSK-3β pathways, resulting in anti-oxidative effects on H9c2 cells that attenuated apoptosis.

Original languageEnglish
Pages (from-to)145-153
Number of pages9
JournalActa Medica Okayama
Volume69
Issue number3
Publication statusPublished - 2015

Fingerprint

Cardiac Myoblasts
Eosinophil Cationic Protein
Glycogen Synthase Kinase 3
Phosphorylation
Oxidative stress
Oxidative Stress
Rats
Cells
Caspase 7
Eosinophils
Cardiac Myocytes
Caspase 3
Reactive Oxygen Species
Cell Survival
Cell Count
Apoptosis

Keywords

  • Akt
  • ECP
  • ERK
  • Reactive oxygen species

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Eosinophil cationic protein shows survival effect on H9c2 cardiac myoblast cells with enhanced phosphorylation of ERK and Akt/GSK-3β under oxidative stress. / Ishii, Hiroko; Kamikawa, Shigeshi; Hirohata, Satoshi; Mizutani, Akifumi; Abe, Koji; Seno, Masaharu; Oohashi, Toshitaka; Ninomiya, Yoshifumi.

In: Acta Medica Okayama, Vol. 69, No. 3, 2015, p. 145-153.

Research output: Contribution to journalArticle

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AU - Mizutani, Akifumi

AU - Abe, Koji

AU - Seno, Masaharu

AU - Oohashi, Toshitaka

AU - Ninomiya, Yoshifumi

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