Enzyme production activity of Phanerochaete chrysosporium and degradation of pentachlorophenol in a bioreactor

Soon Seop Shim, Katsuya Kawamoto

Research output: Contribution to journalArticle

39 Citations (Scopus)


Lignin peroxidase production by a white rot fungus, Phanerochaete chrysosporium, was experimentally investigated using a batch system and a reactor system with various carriers. Immobilization of mycelia cell culture was more effective in promoting cell growth and lignin peroxidase production compared to conventional stationary liquid culture. Biostage carrier, commonly used for biochemical treatment in a fluidized bed disposal system, greatly improved production of lignin peroxidase up to 8.1U/mL in the batch system. The packed bed reactor system was operated using a repeated batch technique, consisting of alternating growth and production phases, to sustain lignin peroxidase growth and production during the entire experiment period. Steady-state continuous PCP degradation over an extended period was accomplished with a mineralization ratio exceeding 80%. These systems and operation methods are promising techniques for the treatment of hazardous waste.

Original languageEnglish
Pages (from-to)4445-4454
Number of pages10
JournalWater Research
Issue number18
Publication statusPublished - Nov 2002
Externally publishedYes



  • Carrier packed-bed reactor system
  • Hazardous waste treatment
  • Lignin peroxidase
  • Pentachlorophenol
  • Phanerochaete chrysosporium

ASJC Scopus subject areas

  • Earth-Surface Processes

Cite this