Enzyme-linked immunosorbent assay of pre-S(2) gene coded polypeptide of hepatitis B virus - correlation of pre-S(2) polypeptide with the receptor for polymerized human serum albumin in sera containing hepatitis B virus surface antigens

Yoshiaki Iwasaki, Hiroshi Ikeda, Kazuhiro Nouso, Kouichi Takaguchi, Takao Tsuji, Kazuharu Matsuura, Takahiro Yamabuki, Masahiko Sawahara

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An enzyme-linked immunosorbent assay(ELISA)was developed to detect polypeptide coded by the pre-S(2)region of hepatitis B virus (HBV) DNA with monoclonal antibody in 120 sera containing hepatitis B virus surface antigen particles. Binding sites for polymerized human serum albumin (pHSA) have been found to be encoded by the pre-S(2) region of hepatitis B virus genome. There can be seen a close correlation between the titers of pre-S (2) polypeptide and those of HBV-pHSA receptor (HBV-pAR) (HBV-asymptomatic carrier : r= 0.826, P<0.01, chronic hepatitis : r=0.787, P< 0.01). The titers of pre-S (2) polypeptide of sera from 75 hepatitis B e antigen (HBeAg) positive carriers (mean±SD) was 92±9 in asymptomatic carrier(ASC), 78±21 in chronic persistent hepatitis (CPH), 66±31 in chronic aggressive hepatitis activity moderate (CAH2A), 44±28 in chronic aggressive hepatitis activity severe (CAH2B), 30±6 in liver cirrhosis (LC), and 21±30 in 15 anti-HBe positive ASC, Under interferon treatment in patient with CAH2B, the titer of pre-S (2) polypeptide as well as HBV-pAR activity was significantly reduced before HBeAg was reduced and the serum transaminase was normalized. These results suggest that the ELISA described for detection of pre-S(2) polypeptide might be useful as a prognostic test for HBV-carriers.

Original languageEnglish
Pages (from-to)593-598
Number of pages6
JournalJapanese Journal of Clinical Immunology
Volume11
Issue number6
DOIs
Publication statusPublished - 1988

Fingerprint

Hepatitis B Surface Antigens
Hepatitis B virus
Serum Albumin
Enzyme-Linked Immunosorbent Assay
Peptides
Chronic Hepatitis
Serum
Genes
Hepatitis B e Antigens
Albumin Receptors
Transaminases
polyalbumin
Liver Cirrhosis
Interferons
Binding Sites
Monoclonal Antibodies
Genome
DNA

Keywords

  • albumin receptor
  • anti-pre-S(2)
  • HBV-pHSA receptor
  • hepatits B virus
  • pre-S(2) antigen

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Enzyme-linked immunosorbent assay of pre-S(2) gene coded polypeptide of hepatitis B virus - correlation of pre-S(2) polypeptide with the receptor for polymerized human serum albumin in sera containing hepatitis B virus surface antigens. / Iwasaki, Yoshiaki; Ikeda, Hiroshi; Nouso, Kazuhiro; Takaguchi, Kouichi; Tsuji, Takao; Matsuura, Kazuharu; Yamabuki, Takahiro; Sawahara, Masahiko.

In: Japanese Journal of Clinical Immunology, Vol. 11, No. 6, 1988, p. 593-598.

Research output: Contribution to journalArticle

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abstract = "An enzyme-linked immunosorbent assay(ELISA)was developed to detect polypeptide coded by the pre-S(2)region of hepatitis B virus (HBV) DNA with monoclonal antibody in 120 sera containing hepatitis B virus surface antigen particles. Binding sites for polymerized human serum albumin (pHSA) have been found to be encoded by the pre-S(2) region of hepatitis B virus genome. There can be seen a close correlation between the titers of pre-S (2) polypeptide and those of HBV-pHSA receptor (HBV-pAR) (HBV-asymptomatic carrier : r= 0.826, P<0.01, chronic hepatitis : r=0.787, P< 0.01). The titers of pre-S (2) polypeptide of sera from 75 hepatitis B e antigen (HBeAg) positive carriers (mean±SD) was 92±9 in asymptomatic carrier(ASC), 78±21 in chronic persistent hepatitis (CPH), 66±31 in chronic aggressive hepatitis activity moderate (CAH2A), 44±28 in chronic aggressive hepatitis activity severe (CAH2B), 30±6 in liver cirrhosis (LC), and 21±30 in 15 anti-HBe positive ASC, Under interferon treatment in patient with CAH2B, the titer of pre-S (2) polypeptide as well as HBV-pAR activity was significantly reduced before HBeAg was reduced and the serum transaminase was normalized. These results suggest that the ELISA described for detection of pre-S(2) polypeptide might be useful as a prognostic test for HBV-carriers.",
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AU - Ikeda, Hiroshi

AU - Nouso, Kazuhiro

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AU - Matsuura, Kazuharu

AU - Yamabuki, Takahiro

AU - Sawahara, Masahiko

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