Enzyme-linked immunosorbent assay for screening of canine brucellosis using recombinant Cu-Zn superoxide dismutase

Ganbat Tsogtbaatar, Masato Tachibana, Kenta Watanabe, Suk Kim, Hiroshi Suzuki, Masahisa Watarai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods including agglutination and gel diffusion tests. In this study, recombinant B. canis Cu-Zn superoxide dismutase (SOD) was used as an antigen for the enzyme-linked immunosorbent assay (ELISA). The recombinant SOD showed a specific reaction with serum infected with B. canis in Western blotting and ELISA. These results suggest that ELISA using recombinant SOD is useful in screening for canine brucellosis.

Original languageEnglish
Pages (from-to)1387-1389
Number of pages3
JournalJournal of Veterinary Medical Science
Volume70
Issue number12
DOIs
Publication statusPublished - Dec 2008
Externally publishedYes

Fingerprint

Brucella canis
Brucella melitensis biovar Canis
Brucellosis
brucellosis
Canidae
superoxide dismutase
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
screening
Superoxide Dismutase
dogs
Immunodiffusion
Agglutination
agglutination
Western blotting
Western Blotting
gels
antigens
Antigens
pathogens

Keywords

  • Brucellosis
  • Cu-Zn superoxide dismutase
  • ELISA

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Enzyme-linked immunosorbent assay for screening of canine brucellosis using recombinant Cu-Zn superoxide dismutase. / Tsogtbaatar, Ganbat; Tachibana, Masato; Watanabe, Kenta; Kim, Suk; Suzuki, Hiroshi; Watarai, Masahisa.

In: Journal of Veterinary Medical Science, Vol. 70, No. 12, 12.2008, p. 1387-1389.

Research output: Contribution to journalArticle

Tsogtbaatar, Ganbat ; Tachibana, Masato ; Watanabe, Kenta ; Kim, Suk ; Suzuki, Hiroshi ; Watarai, Masahisa. / Enzyme-linked immunosorbent assay for screening of canine brucellosis using recombinant Cu-Zn superoxide dismutase. In: Journal of Veterinary Medical Science. 2008 ; Vol. 70, No. 12. pp. 1387-1389.
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