Enzyme-linked immunosorbent assay for human pulmonary surfactant protein D

Takeshi Inoue, Eiji Matsuura, Atsuo Nagata, Yoshinori Ogasawara, Akiko Hattori, Kuriki Yoshio, Seiichiro Fujimoto, Toyoaki Akino

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51 Citations (Scopus)

Abstract

We developed a simple and sensitive enzyme-linked immunosorbent assay (ELISA) for human pulmonary surfactant protein D (SP-D). Human SP-D was purified from bronchoalveolar lavage fluids of patients with pulmonary alveolar proteinosis. Nine monoclonal antibodies (MAbs) were established from BALB/c mice immunized of patients with pulmonary alveolar proteinosis or in amniotic fluids from healthy normal pregnancies. The ELISA is based on a sandwich method using two MAbs, 6B2 and 7C6. Cross-reactivity to human SP-A or rat SP-D was evaluated as below 0.6%. The recovery of different concentrations of SP-D ranged from 94.4% to 111.2%, and serial dilutions of amniotic fluids showed good linearity. SP-D concentrations in 21 amniotic fluids from normal pregnancies were measured by the ELISA. The mean concentrations in amniotic fluids pregnancies in the third trimester was significantly higher than that from earlier stages of gestation (p < 0.001), indicating that this ELISA may be applicable for prediction of fetal lung maturity.

Original languageEnglish
Pages (from-to)157-164
Number of pages8
JournalJournal of Immunological Methods
Volume173
Issue number2
DOIs
Publication statusPublished - Aug 1 1994
Externally publishedYes

Keywords

  • Monoclonal antibody
  • Sandwich ELISA
  • Surfactant protein D

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Inoue, T., Matsuura, E., Nagata, A., Ogasawara, Y., Hattori, A., Yoshio, K., Fujimoto, S., & Akino, T. (1994). Enzyme-linked immunosorbent assay for human pulmonary surfactant protein D. Journal of Immunological Methods, 173(2), 157-164. https://doi.org/10.1016/0022-1759(94)90295-X