Enzyme Immunoassay of a Substance P-like Immunoreactive Substance in Human Plasma and Saliva

Masaharu Takeyama, Kazuo Mori, Fusako Takayama, Kazuhiro Kondo, Kouki Kitagawa

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A sensitive and specific double-antibody enzyme immunoassay (EIA) for a substance P (SP)-like immunoreactive substance (SP-IS) was developed. For competitive reactions, the SP-antibody was incubated with SP standard (or sample) and β-D-galactosidase labeled Tyr8-SP (delayed addition). Free and antibody-bound enzyme hapten were separated by using an anti-rabbit immunoglobulin G coated immunoplate. Activity of the enzyme on the plate was fluorometrically determined. The present immunoassay allows detection of 0.4 to l0fmol/well of SP. Using the present EIA, SP-ISs in human saliva and plasma were determined. The level of SP-lS in human saliva was about 7 pmol/1, which was almost three times higher than that in human plasma.

Original languageEnglish
Pages (from-to)3494-3496
Number of pages3
JournalChemical and Pharmaceutical Bulletin
Volume38
Issue number12
DOIs
Publication statusPublished - 1990
Externally publishedYes

Keywords

  • Tyr-substance P-labeled-α-D-galactosidasc
  • fluorogenic substrate
  • highly sensitive enzyme immunoassay
  • human plasma
  • human saliva
  • immunoreactive substance
  • substance P-like

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

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