Enzyme immunoassay for mabuterol, a selective °2-adrenergic stimulant in the trachea

Itaru Yamamoto, Eiji Matsuura, Masahiro Horiba, Kazuo Akima, Kazuo Nomura, Tetsushi Aizawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A sensitive double antibody and heterologous enzyme immunoassay for mabuterol was established. For competitive reactions, antibody raised against diazotized mabuterol-human serum albumin was incubated with a mixture of diazotized mabuterol analog (RC-1) labeled with (°-D-galactosidase and standard or sample. Free and antibody-bound enzyme hapten were separated using anti-rabbit IgG immobilized on polystyrene balls. Activity of the enzyme on the solid phase was fluorometrically determined. The present immunoassay allows detection of 0.5 to 100 pg/tube of mabuterol. Pharmacokinetic behavior of this agent in human plasma and urine was studied after a single oral administration (50 μg). The maximum level was achieved after 2–3 hrs with approximately 280 pg mabuterol /m1 of plasma and the half life of mabuterol was estimated to be 19.5 hrs. Cumulative amount of mabuterol in the first 72 hrs urine was 64.3 ± 13.2% of the administered dose.

Original languageEnglish
Pages (from-to)261-276
Number of pages16
JournalJournal of Immunoassay
Volume6
Issue number3
DOIs
Publication statusPublished - Sep 1 1985

Keywords

  • enzyme immunoassay
  • mabuterol (KF-868)
  • polystyrene ball
  • °-adrenergic stimulant

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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