Enzymatic properties of endo-β-N-acetylglucosaminidases from developing tomato fruits and soybean seeds: substrate specificity of plant origin endoglycosidase

Yoshinobu Kimura, Toshihiko Tokuda, Akira Ohno, Hiroshi Tanaka, Yukio Ishiguro

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Substrate specificity and some other enzymatic properties of partial purified endo-β-N-acetylglucosaminidases (endo-β-GlcNAc-ase) from developing soybean seeds (Glycine max, Endo-GM) and developing tomato fruits (Lycopersicum esculentum, Endo-LE) were studied. The substrate specificity of these two endoglycosidases was explored and compared with regard to various pyridylaminated N-glycans derived from some naturally occurring glycoproteins. For Endo-GM and Endo-LE, several high mannose-type sugar chains bearing α1-2 mannosyl residue(s), Man9-6GlcNAc2-PA (PA is pyridylamino) (80-100% relative hydrolysis), were most favored substrates followed by Man5GlcNAc2-PA (32% for Endo-LE, 43% for Endo-GM), a typical hybrid-type structure (GlcNAc1Man5GlcNAc2-PA; 34% for Endo-LE, 37% for Endo-GM), and then the common core pentasaccharide of N-glycan (Man3GlcNAc2-PA; 9% for Endo-GM and 16% for Endo-LE). On the contrary, both Endo-GM and Endo-LE could barely hydrolyze the xylose-containing N-glycans (Man3Xyl1GlcNAc2-PA, Man3Fuc1Xyl1GlcNAc2-PA) found ubiquitously in plant cells. The molecular mass of these two endoglycosidases was approximately 62 kDa by gel filtration and both Endo-GM and Endo-LE showed maximal activities for Man6GlcNAc2-PA in a weak acidic region (pH 6.0-6.5). Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)27-36
Number of pages10
JournalBiochimica et Biophysica Acta - General Subjects
Volume1381
Issue number1
DOIs
Publication statusPublished - Jun 5 1998

Keywords

  • Endo-β-N-acetylglucosaminidase
  • Glycine max
  • Lycopersicum esculentum
  • N-Glycan metabolism
  • Plant glycoprotein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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