Abstract
The cell-free extract of Streptomyces sp. KO-2388, an albonoursin- producing strain, was found to catalyze the conversion of cyclo(L-Phe-L-Leu) to albonoursin. The conversion activity was simply determined by measuring the increase in ultraviolet (UV) absorption of the reaction mixture at 317 nm, λ(max) (ε 25,400) of albonoursin, where cyclo(L-Phe-L-Leu) had no absorption. The optimum pH and temperature of this bioconversion using the cell-free extract were determined to be pH 8.0-9.5 and 60°C, respectively. Under the optimum conditions, 620 mg/l of albonoursin was obtained with a conversion ratio of 62% after 24 h incubation.
| Original language | English |
|---|---|
| Pages (from-to) | 265-270 |
| Number of pages | 6 |
| Journal | Journal of Molecular Catalysis - B Enzymatic |
| Volume | 6 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 11 1999 |
Keywords
- Bioconversion
- Cyclic dipeptide
- Dehydrodiketopiprazines
- Dioxopiperazine
- Piprazinedione
ASJC Scopus subject areas
- Catalysis
- Bioengineering
- Biochemistry
- Process Chemistry and Technology
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Cite this
Kanzaki, H., Imura, D., Nitoda, T., & Kawazu, K. (1999). Enzymatic dehydrogenation of cyclo(L-Phe-L-Leu) to a bioactive derivative, albonoursin. Journal of Molecular Catalysis - B Enzymatic, 6(3), 265-270. https://doi.org/10.1016/S1381-1177(98)00079-4