Enzymatic dehydrogenation of cyclo(L-Phe-L-Leu) to a bioactive derivative, albonoursin

Hiroshi Kanzaki, Daisuke Imura, Teruhiko Nitoda, Kazuyoshi Kawazu

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


The cell-free extract of Streptomyces sp. KO-2388, an albonoursin- producing strain, was found to catalyze the conversion of cyclo(L-Phe-L-Leu) to albonoursin. The conversion activity was simply determined by measuring the increase in ultraviolet (UV) absorption of the reaction mixture at 317 nm, λ(max) (ε 25,400) of albonoursin, where cyclo(L-Phe-L-Leu) had no absorption. The optimum pH and temperature of this bioconversion using the cell-free extract were determined to be pH 8.0-9.5 and 60°C, respectively. Under the optimum conditions, 620 mg/l of albonoursin was obtained with a conversion ratio of 62% after 24 h incubation.

Original languageEnglish
Pages (from-to)265-270
Number of pages6
JournalJournal of Molecular Catalysis - B Enzymatic
Issue number3
Publication statusPublished - Mar 11 1999


  • Bioconversion
  • Cyclic dipeptide
  • Dehydrodiketopiprazines
  • Dioxopiperazine
  • Piprazinedione

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology


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