We developed a method for screening cyclic dipeptide-producing microbes by enzymatic conversion. In this method, cyclic dipeptides are detected by the combination of: (i) conversion of cyclic dipeptides to dehydro cyclic dipeptides by cyclo(Leu-Phe) oxidase and (ii) detection of the dehydro derivative by UV spectrophotometry using TLC or HPLC analysis based on the absorbance change caused by the conversion. Using this method, the actinomycete strain A8 was isolated as a cyclic dipeptide-producing strain. The cyclic dipeptides were purified from the microbial extract by enzymatic detection-guided fractionation, and their structures were determined to be cyclo(l-Phe-l-Pro) and cyclo(l-Pro-l-Tyr) by spectroscopic methods.
- Cyclo(Leu-Phe) oxidase
- Dehydro cyclic dipeptide
- Enzymatic conversion-based detection
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience