Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan

Muhammad Jahangir Alam, Ken Ichi Tomochika, Shin Ichi Miyoshi, Sumio Shinoda

Research output: Contribution to journalArticlepeer-review

100 Citations (Scopus)

Abstract

Seawater and organic material (live and/or dead matter deposited on any substratum submersed in seawater) were collected during the cool weather season from a coast of the Seto-Inland Sea, Japan, and analyzed to determine Vibrio parahaemolyticus densities and the occurrence of pathogenic strains, defined as those possessing tdh and/or trh genes by the polymerase chain reaction (PCR), using isolated DNA from enrichment culture of the samples. About 95% of the samples were positive for V. parahaemolyticus (with densities of 3 to >1400 cells per 100 ml water or 10 g organic samples) by the most-probable-number (MPN)-PCR technique with species-specific toxR primers, but only 40% were positive by the conventional MPN-culture technique (with densities ranging from 3 to 240 cells per 100 ml water or 10 g organics). Furthermore, the tdh and trh genes were positive in 55% and 20% of samples, respectively, by the MPN-PCR technique. No tdh and trh gene-positive strains were isolated by the conventional MPN-culture procedure. The difference in detection between the MPN-culture and the MPN-PCR techniques appeared to be significant and may be attributed to different detection sensitivities and other factors.

Original languageEnglish
Pages (from-to)83-87
Number of pages5
JournalFEMS Microbiology Letters
Volume208
Issue number1
DOIs
Publication statusPublished - Feb 19 2002

Keywords

  • Ecology of Vibrio
  • ToxR, tdh and trh genes
  • Vibrio parahaemolyticus

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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