Enterotoxic activity of Klebsiella oxytoca cytotoxin in rabbit intestinal loops

J. Minami, S. I. Katayama, Osamu Matsushita, H. Sakamoto, A. Okabe

Research output: Contribution to journalArticle

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Abstract

We examined the enterotoxicity of a Klebsiella oxytoca cytotoxin which is produced by K. oxytoca OK-1, a strain from a patient with antibiotic- associated hemorrhagic colitis. Injection of the cytotoxin into ligated ileal and colonic loops in rabbits caused the accumulation of fluid in the loops. The fluid was bloody in the ileal loops but not in the colonic ones. Histological examination revealed intense mucosal hemorrhage with erosion in the ileum, whereas no microscopic change was noted in the colon. The fluid accumulation was shown to be a dose-dependent response in both ileal and colonic loops. The amounts of the cytotoxin required for maximal fluid accumulation in ileal and colonic loops were 60 and 10 μg, respectively. Fluid accumulation was first noticeable in ileal loops 12 h and in colonic ones 5 h after the injection of these doses of the cytotoxin and then proceeded with time. When K. oxytoca OK-1, a cytotoxin-producing strain, was inoculated into the loops at doses of 1 x 108 and 5 x 109 CFU, similar fluid accumulation was observed. However, inoculation of K. oxytoca ATCC 13182, a non-cytotoxin-producing strain, at the same doses did not cause any change. These results suggest that the cytotoxin-producing strain of K. oxytoca is the causative organism of antibiotic-associated hemorrhagic colitis and that the toxin is the factor responsible for pathogenesis.

Original languageEnglish
Pages (from-to)172-177
Number of pages6
JournalInfection and Immunity
Volume62
Issue number1
Publication statusPublished - 1994
Externally publishedYes

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Klebsiella oxytoca
Cytotoxins
Rabbits
Pseudomembranous Enterocolitis
Injections
Ileum
Colon
Hemorrhage

ASJC Scopus subject areas

  • Immunology

Cite this

Minami, J., Katayama, S. I., Matsushita, O., Sakamoto, H., & Okabe, A. (1994). Enterotoxic activity of Klebsiella oxytoca cytotoxin in rabbit intestinal loops. Infection and Immunity, 62(1), 172-177.

Enterotoxic activity of Klebsiella oxytoca cytotoxin in rabbit intestinal loops. / Minami, J.; Katayama, S. I.; Matsushita, Osamu; Sakamoto, H.; Okabe, A.

In: Infection and Immunity, Vol. 62, No. 1, 1994, p. 172-177.

Research output: Contribution to journalArticle

Minami, J, Katayama, SI, Matsushita, O, Sakamoto, H & Okabe, A 1994, 'Enterotoxic activity of Klebsiella oxytoca cytotoxin in rabbit intestinal loops', Infection and Immunity, vol. 62, no. 1, pp. 172-177.
Minami, J. ; Katayama, S. I. ; Matsushita, Osamu ; Sakamoto, H. ; Okabe, A. / Enterotoxic activity of Klebsiella oxytoca cytotoxin in rabbit intestinal loops. In: Infection and Immunity. 1994 ; Vol. 62, No. 1. pp. 172-177.
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