Enhancement of vascular permeability due to histamine-releasing effect of Vibrio vulnificus protease in rat skin

Shin-ichi Miyoshi, Katsumi Sugiyama, Yukio Suzuki, Hiroaki Furuta, Noriko Miyoshi, Sumio Shinoda

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Vibrio vulnificus protease, which had been purified from the culture supernatant of a strain isolated from a septicemic human patient, enhanced hypodermic vascular permeability and formed hemorrhagic spot caused by tissue destruction in a dose-dependent manner within a range of 0.3-10.0 μg when injected intradermally (i.d.) into rat dorsal skin. Enhancement of permeability was observed within 2 min, and the permeability-enhancing reaction terminated at 10 min after the injection. This reaction was blocked by simultaneous injection of an antihistamine, diphenhydramine, suggesting that the permeability-enhancing reaction of V. vulnificus protease was due to histamine release in vivo. Hemorrhage caused by V. vulnificus protease was also rapidly observed, but this reaction was not inhibited by the antihistamine.

Original languageEnglish
Pages (from-to)95-98
Number of pages4
JournalFEMS Microbiology Letters
Issue number1
Publication statusPublished - 1987



  • (Vascular permeability enhancement)
  • Histamine
  • Protease
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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