TY - JOUR
T1 - Enhancement of regulatory T cell-like suppressive function in MT-2 by long-term and low-dose exposure to asbestos
AU - Ying, Chen
AU - Maeda, Megumi
AU - Nishimura, Yasumitsu
AU - Kumagai-Takei, Naoko
AU - Hayashi, Hiroaki
AU - Matsuzaki, Hidenori
AU - Lee, Suni
AU - Yoshitome, Kei
AU - Yamamoto, Shoko
AU - Hatayama, Tamayo
AU - Otsuki, Takemi
N1 - Funding Information:
This study was supported by Special Coordination Funds for Promoting Science and Technology grant H18-1-3-3-1 (Comprehensive Approach on Asbestos-Related Diseases), grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan ( 18390186 , 1965153 , 19790411 , 20390178 and 22700933 ), Ryobi Teien Memory Foundation, The Promotion and Mutual Aid Corporation for Private Schools of Japan, Kawasaki Medical School Project Grants (21-201), the Strategic Research Foundation Grant-aided Project for Private Universities from the Ministry of Education, Culture, Sport, Science, and Technology, Japan, and the Program to Disseminate Tenure Tracking System (FY 2011-2013) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
Publisher Copyright:
© 2015 Elsevier Ireland Ltd.
PY - 2015/12/2
Y1 - 2015/12/2
N2 - Asbestos exposure causes lung fibrosis and various malignant tumors such as lung cancer and malignant mesothelioma. The effects of asbestos on immune cells have not been thoroughly investigated, although our previous reports showed that asbestos exposure reduced anti-tumor immunity. The effects of continuous exposure of regulatory T cells (Treg) to asbestos were examined using the HTLV-1 immortalized human T cell line MT-2, which possesses a suppressive function and expresses the Treg marker protein, Foxp3. Sublines were generated by the continuous exposure to low doses of asbestos fibers for more than one year. The sublines exposed to asbestos showed enhanced suppressive Treg function via cell-cell contact, and increased production of soluble factors such as IL-10 and transforming growth factor (TGF)-β1. These results also indicated that asbestos exposure induced the reduction of anti-tumor immunity, and efforts to develop substances to reverse this reduction may be helpful in preventing the occurrence of asbestos-induced tumors.
AB - Asbestos exposure causes lung fibrosis and various malignant tumors such as lung cancer and malignant mesothelioma. The effects of asbestos on immune cells have not been thoroughly investigated, although our previous reports showed that asbestos exposure reduced anti-tumor immunity. The effects of continuous exposure of regulatory T cells (Treg) to asbestos were examined using the HTLV-1 immortalized human T cell line MT-2, which possesses a suppressive function and expresses the Treg marker protein, Foxp3. Sublines were generated by the continuous exposure to low doses of asbestos fibers for more than one year. The sublines exposed to asbestos showed enhanced suppressive Treg function via cell-cell contact, and increased production of soluble factors such as IL-10 and transforming growth factor (TGF)-β1. These results also indicated that asbestos exposure induced the reduction of anti-tumor immunity, and efforts to develop substances to reverse this reduction may be helpful in preventing the occurrence of asbestos-induced tumors.
KW - Asbestos
KW - CTLA-4
KW - IL-10
KW - Regulatory T cell
KW - TGF-β1
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U2 - 10.1016/j.tox.2015.10.005
DO - 10.1016/j.tox.2015.10.005
M3 - Article
C2 - 26505785
AN - SCOPUS:84945536804
VL - 338
SP - 86
EP - 94
JO - Toxicology
JF - Toxicology
SN - 0300-483X
ER -
