Enhancement of nitrate efflux and the plasma membrane hydrogen ion-transport activity of barley roots by nitrate treatment

Kousei Yamashita, Minobu Kasai, Yoko Yamamoto, Hideaki Matsumoto

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of nitrate (NO3) on the activities of hydrogen (H+)-ATPase and H+-transport in the plasma membrane vesicles isolated from barley (Hordeum vulgare L. cv. Kikaihadaka) roots was investigated. After treatment with 10 mM NO3 for longer than 24 h, both activities of H+-ATPase and H+-transport assayed in the presence of chloride (Cl) were stimulated less than 20%. While H+-transport activity was approximately two times higher in NO3-grown roots than in control roots when assayed in the presence of NO3 instead of Cl. This result suggests that NO3 is permeable in the plasma membrane vesicles isolated from NO3-grown roots rather than that of the control, and thereby stimulate H+-transport activity through the collapse of positive potentials established by H+-ATPase. Furthermore, NO3 efflux from roots was markedly enhanced after 48 h of the exposure to NO3. Taken together, NO3 efflux may be mediated by the permeability to NOD which is induced by NO3.

Original languageEnglish
Pages (from-to)657-668
Number of pages12
JournalJournal of Plant Nutrition
Volume20
Issue number6
Publication statusPublished - 1997

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hydrogen ions
H-transporting ATP synthase
Proton-Translocating ATPases
Ion Transport
ion transport
Hordeum
Nitrates
Protons
plasma membrane
barley
nitrates
Cell Membrane
Chlorides
chlorides
Hordeum vulgare
hydrogen
Hydrogen
Permeability
permeability

ASJC Scopus subject areas

  • Plant Science

Cite this

Enhancement of nitrate efflux and the plasma membrane hydrogen ion-transport activity of barley roots by nitrate treatment. / Yamashita, Kousei; Kasai, Minobu; Yamamoto, Yoko; Matsumoto, Hideaki.

In: Journal of Plant Nutrition, Vol. 20, No. 6, 1997, p. 657-668.

Research output: Contribution to journalArticle

Yamashita, Kousei ; Kasai, Minobu ; Yamamoto, Yoko ; Matsumoto, Hideaki. / Enhancement of nitrate efflux and the plasma membrane hydrogen ion-transport activity of barley roots by nitrate treatment. In: Journal of Plant Nutrition. 1997 ; Vol. 20, No. 6. pp. 657-668.
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N2 - The effect of nitrate (NO3) on the activities of hydrogen (H+)-ATPase and H+-transport in the plasma membrane vesicles isolated from barley (Hordeum vulgare L. cv. Kikaihadaka) roots was investigated. After treatment with 10 mM NO3 for longer than 24 h, both activities of H+-ATPase and H+-transport assayed in the presence of chloride (Cl) were stimulated less than 20%. While H+-transport activity was approximately two times higher in NO3-grown roots than in control roots when assayed in the presence of NO3 instead of Cl. This result suggests that NO3 is permeable in the plasma membrane vesicles isolated from NO3-grown roots rather than that of the control, and thereby stimulate H+-transport activity through the collapse of positive potentials established by H+-ATPase. Furthermore, NO3 efflux from roots was markedly enhanced after 48 h of the exposure to NO3. Taken together, NO3 efflux may be mediated by the permeability to NOD which is induced by NO3.

AB - The effect of nitrate (NO3) on the activities of hydrogen (H+)-ATPase and H+-transport in the plasma membrane vesicles isolated from barley (Hordeum vulgare L. cv. Kikaihadaka) roots was investigated. After treatment with 10 mM NO3 for longer than 24 h, both activities of H+-ATPase and H+-transport assayed in the presence of chloride (Cl) were stimulated less than 20%. While H+-transport activity was approximately two times higher in NO3-grown roots than in control roots when assayed in the presence of NO3 instead of Cl. This result suggests that NO3 is permeable in the plasma membrane vesicles isolated from NO3-grown roots rather than that of the control, and thereby stimulate H+-transport activity through the collapse of positive potentials established by H+-ATPase. Furthermore, NO3 efflux from roots was markedly enhanced after 48 h of the exposure to NO3. Taken together, NO3 efflux may be mediated by the permeability to NOD which is induced by NO3.

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