Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces

Hiroyuki Ijima, Sunao Murakami, Takeshi Matsuo, Takayuki Takei, Tsutomu Ono, Koei Kawakami

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Primary rat hepatocytes were co-cultured with bone marrow cells (BMCs) in a tissue culture-treated culture well (TCW) or a collagen-coated culture well (CCW). Although a medium containing serum was used, the co-cultured cells formed spheroids on the TCW within 2 days, and ammonia metabolism and albumin secretion activities were well maintained for 3 weeks. The co-cultured cells formed a monolayer on the CCW within 2 days, and liver functions were maintained for 3 weeks. The ammonia metabolism activities and albumin secretion activities of co-cultured cells on the CCWs were slightly inferior to those on the TCWs. This co-culture system of hepatocytes and BMCs is an appropriate culture system for the expression and maintenance of liver-specific functions in vitro, and is expected to be applicable to bioartificial liver systems, regenerative medicine, and liver function simulators.

Original languageEnglish
Pages (from-to)104-109
Number of pages6
JournalJournal of Artificial Organs
Volume8
Issue number2
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

Fingerprint

Tissue culture
Polystyrenes
Bone Marrow Cells
Liver
Rats
Hepatocytes
Cultured Cells
Bone
Cells
Ammonia
Albumins
Cell culture
Metabolism
Artificial Liver
Regenerative Medicine
Coculture Techniques
Systems Analysis
Collagen
Maintenance
Monolayers

Keywords

  • Albumin secretion
  • Ammonia metabolism
  • Bone marrow cells
  • Co-culture
  • Hepatocyte

ASJC Scopus subject areas

  • Biophysics

Cite this

Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces. / Ijima, Hiroyuki; Murakami, Sunao; Matsuo, Takeshi; Takei, Takayuki; Ono, Tsutomu; Kawakami, Koei.

In: Journal of Artificial Organs, Vol. 8, No. 2, 08.2005, p. 104-109.

Research output: Contribution to journalArticle

Ijima, Hiroyuki ; Murakami, Sunao ; Matsuo, Takeshi ; Takei, Takayuki ; Ono, Tsutomu ; Kawakami, Koei. / Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces. In: Journal of Artificial Organs. 2005 ; Vol. 8, No. 2. pp. 104-109.
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