Abstract
Primary rat hepatocytes were co-cultured with bone marrow cells (BMCs) in a tissue culture-treated culture well (TCW) or a collagen-coated culture well (CCW). Although a medium containing serum was used, the co-cultured cells formed spheroids on the TCW within 2 days, and ammonia metabolism and albumin secretion activities were well maintained for 3 weeks. The co-cultured cells formed a monolayer on the CCW within 2 days, and liver functions were maintained for 3 weeks. The ammonia metabolism activities and albumin secretion activities of co-cultured cells on the CCWs were slightly inferior to those on the TCWs. This co-culture system of hepatocytes and BMCs is an appropriate culture system for the expression and maintenance of liver-specific functions in vitro, and is expected to be applicable to bioartificial liver systems, regenerative medicine, and liver function simulators.
| Original language | English |
|---|---|
| Pages (from-to) | 104-109 |
| Number of pages | 6 |
| Journal | Journal of Artificial Organs |
| Volume | 8 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 2005 |
| Externally published | Yes |
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Keywords
- Albumin secretion
- Ammonia metabolism
- Bone marrow cells
- Co-culture
- Hepatocyte
ASJC Scopus subject areas
- Biophysics
Cite this
Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces. / Ijima, Hiroyuki; Murakami, Sunao; Matsuo, Takeshi; Takei, Takayuki; Ono, Tsutomu; Kawakami, Koei.
In: Journal of Artificial Organs, Vol. 8, No. 2, 08.2005, p. 104-109.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces
AU - Ijima, Hiroyuki
AU - Murakami, Sunao
AU - Matsuo, Takeshi
AU - Takei, Takayuki
AU - Ono, Tsutomu
AU - Kawakami, Koei
PY - 2005/8
Y1 - 2005/8
N2 - Primary rat hepatocytes were co-cultured with bone marrow cells (BMCs) in a tissue culture-treated culture well (TCW) or a collagen-coated culture well (CCW). Although a medium containing serum was used, the co-cultured cells formed spheroids on the TCW within 2 days, and ammonia metabolism and albumin secretion activities were well maintained for 3 weeks. The co-cultured cells formed a monolayer on the CCW within 2 days, and liver functions were maintained for 3 weeks. The ammonia metabolism activities and albumin secretion activities of co-cultured cells on the CCWs were slightly inferior to those on the TCWs. This co-culture system of hepatocytes and BMCs is an appropriate culture system for the expression and maintenance of liver-specific functions in vitro, and is expected to be applicable to bioartificial liver systems, regenerative medicine, and liver function simulators.
AB - Primary rat hepatocytes were co-cultured with bone marrow cells (BMCs) in a tissue culture-treated culture well (TCW) or a collagen-coated culture well (CCW). Although a medium containing serum was used, the co-cultured cells formed spheroids on the TCW within 2 days, and ammonia metabolism and albumin secretion activities were well maintained for 3 weeks. The co-cultured cells formed a monolayer on the CCW within 2 days, and liver functions were maintained for 3 weeks. The ammonia metabolism activities and albumin secretion activities of co-cultured cells on the CCWs were slightly inferior to those on the TCWs. This co-culture system of hepatocytes and BMCs is an appropriate culture system for the expression and maintenance of liver-specific functions in vitro, and is expected to be applicable to bioartificial liver systems, regenerative medicine, and liver function simulators.
KW - Albumin secretion
KW - Ammonia metabolism
KW - Bone marrow cells
KW - Co-culture
KW - Hepatocyte
UR - http://www.scopus.com/inward/record.url?scp=23744507498&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=23744507498&partnerID=8YFLogxK
U2 - 10.1007/s10047-005-0289-9
DO - 10.1007/s10047-005-0289-9
M3 - Article
VL - 8
SP - 104
EP - 109
JO - Journal of Artificial Organs
JF - Journal of Artificial Organs
SN - 1434-7229
IS - 2
ER -