Enhancement of cell-based therapeutic angiogenesis using a novel type of injectable scaffolds of hydroxyapatite-polymer nanocomposite microspheres

Yohei Mima, Shinya Fukumoto, Hidenori Koyama, Masahiro Okada, Shinji Tanaka, Tetsuo Shoji, Masanori Emoto, Tsutomu Furuzono, Yoshiki Nishizawa, Masaaki Inaba

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Background: Clinical trials demonstrate the effectiveness of cell-based therapeutic angiogenesis in patients with severe ischemic diseases; however, their success remains limited. Maintaining transplanted cells in place are expected to augment the cell-based therapeutic angiogenesis. We have reported that nano-hydroxyapatite (HAp) coating on medical devices shows marked cell adhesiveness. Using this nanotechnology, HAp-coated poly(L-lactic acid) (PLLA) microspheres, named nano-scaffold (NS), were generated as a non-biological, biodegradable and injectable cell scaffold. We investigate the effectiveness of NS on cell-based therapeutic angiogenesis. Methods and Results: Bone marrow mononuclear cells (BMNC) and NS or control PLLA microspheres (LA) were intramuscularly co-implanted into mice ischemic hindlimbs. When BMNC derived from enhanced green fluorescent protein (EGFP)-transgenic mice were injected into ischemic muscle, the muscle GFP level in NS+BMNC group was approximate fivefold higher than that in BMNC or LA+BMNC groups seven days after operation. Kaplan-Meier analysis demonstrated that NS+BMNC markedly prevented hindlimb necrosis (P

Original languageEnglish
Article numbere35199
Pages (from-to)e35199-e35199
Number of pages1
JournalPLoS One
Volume7
Issue number4
DOIs
Publication statusPublished - Apr 18 2012
Externally publishedYes

Fingerprint

polymer nanocomposites
Nanocomposites
hydroxyapatite
Durapatite
angiogenesis
Microspheres
Scaffolds
Bone Marrow Cells
Polymers
Bone
therapeutics
Injections
bone marrow
cells
Hindlimb
Muscle
Therapeutics
polylactic acid
Adhesiveness
Muscles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Enhancement of cell-based therapeutic angiogenesis using a novel type of injectable scaffolds of hydroxyapatite-polymer nanocomposite microspheres. / Mima, Yohei; Fukumoto, Shinya; Koyama, Hidenori; Okada, Masahiro; Tanaka, Shinji; Shoji, Tetsuo; Emoto, Masanori; Furuzono, Tsutomu; Nishizawa, Yoshiki; Inaba, Masaaki.

In: PLoS One, Vol. 7, No. 4, e35199, 18.04.2012, p. e35199-e35199.

Research output: Contribution to journalArticle

Mima, Y, Fukumoto, S, Koyama, H, Okada, M, Tanaka, S, Shoji, T, Emoto, M, Furuzono, T, Nishizawa, Y & Inaba, M 2012, 'Enhancement of cell-based therapeutic angiogenesis using a novel type of injectable scaffolds of hydroxyapatite-polymer nanocomposite microspheres', PLoS One, vol. 7, no. 4, e35199, pp. e35199-e35199. https://doi.org/10.1371/journal.pone.0035199
Mima, Yohei ; Fukumoto, Shinya ; Koyama, Hidenori ; Okada, Masahiro ; Tanaka, Shinji ; Shoji, Tetsuo ; Emoto, Masanori ; Furuzono, Tsutomu ; Nishizawa, Yoshiki ; Inaba, Masaaki. / Enhancement of cell-based therapeutic angiogenesis using a novel type of injectable scaffolds of hydroxyapatite-polymer nanocomposite microspheres. In: PLoS One. 2012 ; Vol. 7, No. 4. pp. e35199-e35199.
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