Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression

Masaki Magari, Takahiro Aya, Mika Ikeda, Kagefumi Todo, Naoki Kanayama, Hitoshi Ohmori

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We developed a novel in vitro antibody (Ab) generation system using a hypermutating chicken B cell line (DT40-SW). We suppressed the expression of the Pax5 transcription factor by targeted disruption of the gene to increase Ab production in isolated clones and produce the desired Abs. This single genetic manipulation resulted in a significant enhancement of Ab production without significantly affecting maximum cell density.

Original languageEnglish
Pages (from-to)206-209
Number of pages4
JournalJournal of Bioscience and Bioengineering
Volume107
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Antibodies
Antibody Formation
Chickens
B-Lymphocytes
Cells
Cell Line
Transcription factors
Transcription Factors
Clone Cells
Cell Count
Genes
In Vitro Techniques

Keywords

  • Antibody
  • Chicken B cell line
  • Gene knockout
  • Pax5
  • Plasma cell

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression. / Magari, Masaki; Aya, Takahiro; Ikeda, Mika; Todo, Kagefumi; Kanayama, Naoki; Ohmori, Hitoshi.

In: Journal of Bioscience and Bioengineering, Vol. 107, No. 2, 02.2009, p. 206-209.

Research output: Contribution to journalArticle

Magari, Masaki ; Aya, Takahiro ; Ikeda, Mika ; Todo, Kagefumi ; Kanayama, Naoki ; Ohmori, Hitoshi. / Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression. In: Journal of Bioscience and Bioengineering. 2009 ; Vol. 107, No. 2. pp. 206-209.
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