Enhancement of activity of sol-gel immobilized lipase in organic media by pretreatment with substrate analogues

Shin Ya Furukawa, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

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14 Citations (Scopus)


By performing pretreatment with substrate analogues, an activation of lipase immobilized into organic-inorganic hybrid silicate was attempted in esterification of menthol with butyric acid in organic media. With decreasing water content of immobilized lipase preparations, the activity of immobilized lipases by (-)-menthol pretreatment was enhanced, and the highest activity was obtained at water activity, aw=0.11. However, the pretreatment of immobilized lipases with (-)-menthol could not improve the enantioselectivity. In addition, the activity of pretreated immobilized lipases increased with increasing the chain length of alcohol as a substrate analogue. Consequently, it was found that hydrophobic and bulky alcohol was an effective substrate analogue to activate immobilized lipases.

Original languageEnglish
Pages (from-to)65-70
Number of pages6
JournalJournal of Molecular Catalysis - B Enzymatic
Issue number1-3
Publication statusPublished - Sep 28 2001



  • Esterification
  • Lipase
  • Organic-inorganic hybrid silicate
  • Sol-gel method
  • Substrate analogue

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

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