By performing pretreatment with substrate analogues, an activation of lipase immobilized into organic-inorganic hybrid silicate was attempted in esterification of menthol with butyric acid in organic media. With decreasing water content of immobilized lipase preparations, the activity of immobilized lipases by (-)-menthol pretreatment was enhanced, and the highest activity was obtained at water activity, aw=0.11. However, the pretreatment of immobilized lipases with (-)-menthol could not improve the enantioselectivity. In addition, the activity of pretreated immobilized lipases increased with increasing the chain length of alcohol as a substrate analogue. Consequently, it was found that hydrophobic and bulky alcohol was an effective substrate analogue to activate immobilized lipases.
- Organic-inorganic hybrid silicate
- Sol-gel method
- Substrate analogue
ASJC Scopus subject areas
- Process Chemistry and Technology