Enhancement in transport of a hydrophilic marker through intestinal epithelial cell (Caco-2) monolayer by W/O/W multiple emulsion containing C8TG

Motohiro Shima, Miyono Tanaka, Yukitaka Kimura, Shuji Adachi, Ryuichi Matsuno

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A W/O/W emulsion containing octanoic acid triacylglycerol (C8TG) was prepared as a carrier for hydrophilic bioactive substances. The absorption enhancement effect of the hydrolysates of C8TG in the W/O/W emulsion was investigated by measuring the transport of a hydrophilic marker through the intestinal epithelial (Caco-2) cell monolayer. First, the concentrations of gall and lipase in an artificial digestive fluid, of which the integrity of the Caco-2 monolayer was not affected, were both determined to be 0.1% (w/v). The W/O/W emulsion including the hydrophilic marker molecule was hydrolyzed by the artificial digestive fluid and the apparent permeability of the hydrophilic marker substance was measured. In order to clarify the components affecting the transport enhancement, octanoic acid monoacylglycerol (C8MG) was mixed with a W/O/W emulsion at the same concentration as that in the emulsion hydrolyzed by the digestive fluid. The dependence of the apparent permeability on the C8MG concentration was the same for both emulsions, indicating that C8MG, hydrolyzed from the oil phase of the W/O/W emulsions, played an important role in the transport enhancement effect of the W/O/W emulsions.

Original languageEnglish
Pages (from-to)321-328
Number of pages8
JournalFood Hydrocolloids
Volume19
Issue number2
DOIs
Publication statusPublished - Mar 2005
Externally publishedYes

Fingerprint

Emulsions
emulsions
Monolayers
epithelial cells
Epithelial Cells
octanoic acid
Fluids
Permeability
permeability
Monoglycerides
Caco-2 Cells
monoacylglycerols
Acids
Lipases
galls
Lipase
hydrolysates
Oils
Triglycerides
triacylglycerols

Keywords

  • Caco-2
  • Digestive lipase
  • Release of the hydrophilic marker
  • Transport enhancement
  • W/O/W multiple emulsion

ASJC Scopus subject areas

  • Food Science

Cite this

Enhancement in transport of a hydrophilic marker through intestinal epithelial cell (Caco-2) monolayer by W/O/W multiple emulsion containing C8TG. / Shima, Motohiro; Tanaka, Miyono; Kimura, Yukitaka; Adachi, Shuji; Matsuno, Ryuichi.

In: Food Hydrocolloids, Vol. 19, No. 2, 03.2005, p. 321-328.

Research output: Contribution to journalArticle

@article{12a18e5c53de4e21b56d81cd7a466bb1,
title = "Enhancement in transport of a hydrophilic marker through intestinal epithelial cell (Caco-2) monolayer by W/O/W multiple emulsion containing C8TG",
abstract = "A W/O/W emulsion containing octanoic acid triacylglycerol (C8TG) was prepared as a carrier for hydrophilic bioactive substances. The absorption enhancement effect of the hydrolysates of C8TG in the W/O/W emulsion was investigated by measuring the transport of a hydrophilic marker through the intestinal epithelial (Caco-2) cell monolayer. First, the concentrations of gall and lipase in an artificial digestive fluid, of which the integrity of the Caco-2 monolayer was not affected, were both determined to be 0.1{\%} (w/v). The W/O/W emulsion including the hydrophilic marker molecule was hydrolyzed by the artificial digestive fluid and the apparent permeability of the hydrophilic marker substance was measured. In order to clarify the components affecting the transport enhancement, octanoic acid monoacylglycerol (C8MG) was mixed with a W/O/W emulsion at the same concentration as that in the emulsion hydrolyzed by the digestive fluid. The dependence of the apparent permeability on the C8MG concentration was the same for both emulsions, indicating that C8MG, hydrolyzed from the oil phase of the W/O/W emulsions, played an important role in the transport enhancement effect of the W/O/W emulsions.",
keywords = "Caco-2, Digestive lipase, Release of the hydrophilic marker, Transport enhancement, W/O/W multiple emulsion",
author = "Motohiro Shima and Miyono Tanaka and Yukitaka Kimura and Shuji Adachi and Ryuichi Matsuno",
year = "2005",
month = "3",
doi = "10.1016/j.foodhyd.2004.07.004",
language = "English",
volume = "19",
pages = "321--328",
journal = "Food Hydrocolloids",
issn = "0268-005X",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Enhancement in transport of a hydrophilic marker through intestinal epithelial cell (Caco-2) monolayer by W/O/W multiple emulsion containing C8TG

AU - Shima, Motohiro

AU - Tanaka, Miyono

AU - Kimura, Yukitaka

AU - Adachi, Shuji

AU - Matsuno, Ryuichi

PY - 2005/3

Y1 - 2005/3

N2 - A W/O/W emulsion containing octanoic acid triacylglycerol (C8TG) was prepared as a carrier for hydrophilic bioactive substances. The absorption enhancement effect of the hydrolysates of C8TG in the W/O/W emulsion was investigated by measuring the transport of a hydrophilic marker through the intestinal epithelial (Caco-2) cell monolayer. First, the concentrations of gall and lipase in an artificial digestive fluid, of which the integrity of the Caco-2 monolayer was not affected, were both determined to be 0.1% (w/v). The W/O/W emulsion including the hydrophilic marker molecule was hydrolyzed by the artificial digestive fluid and the apparent permeability of the hydrophilic marker substance was measured. In order to clarify the components affecting the transport enhancement, octanoic acid monoacylglycerol (C8MG) was mixed with a W/O/W emulsion at the same concentration as that in the emulsion hydrolyzed by the digestive fluid. The dependence of the apparent permeability on the C8MG concentration was the same for both emulsions, indicating that C8MG, hydrolyzed from the oil phase of the W/O/W emulsions, played an important role in the transport enhancement effect of the W/O/W emulsions.

AB - A W/O/W emulsion containing octanoic acid triacylglycerol (C8TG) was prepared as a carrier for hydrophilic bioactive substances. The absorption enhancement effect of the hydrolysates of C8TG in the W/O/W emulsion was investigated by measuring the transport of a hydrophilic marker through the intestinal epithelial (Caco-2) cell monolayer. First, the concentrations of gall and lipase in an artificial digestive fluid, of which the integrity of the Caco-2 monolayer was not affected, were both determined to be 0.1% (w/v). The W/O/W emulsion including the hydrophilic marker molecule was hydrolyzed by the artificial digestive fluid and the apparent permeability of the hydrophilic marker substance was measured. In order to clarify the components affecting the transport enhancement, octanoic acid monoacylglycerol (C8MG) was mixed with a W/O/W emulsion at the same concentration as that in the emulsion hydrolyzed by the digestive fluid. The dependence of the apparent permeability on the C8MG concentration was the same for both emulsions, indicating that C8MG, hydrolyzed from the oil phase of the W/O/W emulsions, played an important role in the transport enhancement effect of the W/O/W emulsions.

KW - Caco-2

KW - Digestive lipase

KW - Release of the hydrophilic marker

KW - Transport enhancement

KW - W/O/W multiple emulsion

UR - http://www.scopus.com/inward/record.url?scp=8644255133&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8644255133&partnerID=8YFLogxK

U2 - 10.1016/j.foodhyd.2004.07.004

DO - 10.1016/j.foodhyd.2004.07.004

M3 - Article

VL - 19

SP - 321

EP - 328

JO - Food Hydrocolloids

JF - Food Hydrocolloids

SN - 0268-005X

IS - 2

ER -