Enhanced production of leukotrienes by peripheral leukocytes and specific IgE antibodies in patients with chronic obstructive pulmonary disease

Fumihiro Mitsunobu, Takashi Mifune, Yasuhiro Hosaki, Kozo Ashida, Hirofumi Tsugeno, Makoto Okamoto, Shingo Takata, Yoshiro Tanizaki

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background: How leukotrienes (LTs) and IgE-mediated allergy reflect clinical features in patients with chronic obstructive pulmonary disease (COPD) remains unclear. Objective: Our goal was to determine whether LTB 4 and LTC 4 would correlate with airway obstruction and whether IgE-mediated allergy would influence the generation of LTs and bronchial hyperresponsiveness in patients with COPD. Methods: We measured the pulmonary function, methacholine bronchial hyperresponsiveness, and generation of LTB 4 and LTC 4 from peripheral leukocytes stimulated with calcium ionophore A23187 in relation to the presence of specific IgE antibodies against inhalant allergens. Results: The leukocytes of patients with COPD generated significantly more LTB 4 (with allergy, P <.001; without allergy, P <.001) and LTC 4 (with allergy, P <.001; without allergy, P <.01) than the leukocytes of the control subjects. LTC 4 production was significantly higher in the allergic COPD subjects than in the nonallergic COPD patients (P <.01), but the amount of LTB 4 generated was not significantly different. FEV 1 significantly correlated with the level of both LTB 4 (with allergy, r = -0.556, P = .0375; without allergy, r = -0.731, P = .0046) and LTC 4 (with allergy, r = -0.764, P = .0043; without allergy, r = -0.526, P = .0414) generation in COPD. The log 10 of the minimum dose of methacholine was significantly higher in COPD patients without allergy than in those with allergy (P <.05). Conclusion: Enhanced LT generation from peripheral leukocytes is observed in patients with COPD, and the presence of specific IgE antibodies against inhalant allergens enhances LTC 4 generation, bronchial hyperresponsiveness, and the relationship between LTC 4 generation and airway obstruction.

Original languageEnglish
Pages (from-to)492-498
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume107
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

Leukotrienes
Chronic Obstructive Pulmonary Disease
Immunoglobulin E
Hypersensitivity
Leukocytes
Antibodies
Methacholine Chloride
Airway Obstruction
Allergens
Calcium Ionophores
Calcimycin

Keywords

  • Bronchial hyperresponsiveness
  • COPD
  • IgE-mediated allergy
  • LTB
  • LTC

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Enhanced production of leukotrienes by peripheral leukocytes and specific IgE antibodies in patients with chronic obstructive pulmonary disease. / Mitsunobu, Fumihiro; Mifune, Takashi; Hosaki, Yasuhiro; Ashida, Kozo; Tsugeno, Hirofumi; Okamoto, Makoto; Takata, Shingo; Tanizaki, Yoshiro.

In: Journal of Allergy and Clinical Immunology, Vol. 107, No. 3, 2001, p. 492-498.

Research output: Contribution to journalArticle

Mitsunobu, Fumihiro ; Mifune, Takashi ; Hosaki, Yasuhiro ; Ashida, Kozo ; Tsugeno, Hirofumi ; Okamoto, Makoto ; Takata, Shingo ; Tanizaki, Yoshiro. / Enhanced production of leukotrienes by peripheral leukocytes and specific IgE antibodies in patients with chronic obstructive pulmonary disease. In: Journal of Allergy and Clinical Immunology. 2001 ; Vol. 107, No. 3. pp. 492-498.
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abstract = "Background: How leukotrienes (LTs) and IgE-mediated allergy reflect clinical features in patients with chronic obstructive pulmonary disease (COPD) remains unclear. Objective: Our goal was to determine whether LTB 4 and LTC 4 would correlate with airway obstruction and whether IgE-mediated allergy would influence the generation of LTs and bronchial hyperresponsiveness in patients with COPD. Methods: We measured the pulmonary function, methacholine bronchial hyperresponsiveness, and generation of LTB 4 and LTC 4 from peripheral leukocytes stimulated with calcium ionophore A23187 in relation to the presence of specific IgE antibodies against inhalant allergens. Results: The leukocytes of patients with COPD generated significantly more LTB 4 (with allergy, P <.001; without allergy, P <.001) and LTC 4 (with allergy, P <.001; without allergy, P <.01) than the leukocytes of the control subjects. LTC 4 production was significantly higher in the allergic COPD subjects than in the nonallergic COPD patients (P <.01), but the amount of LTB 4 generated was not significantly different. FEV 1 significantly correlated with the level of both LTB 4 (with allergy, r = -0.556, P = .0375; without allergy, r = -0.731, P = .0046) and LTC 4 (with allergy, r = -0.764, P = .0043; without allergy, r = -0.526, P = .0414) generation in COPD. The log 10 of the minimum dose of methacholine was significantly higher in COPD patients without allergy than in those with allergy (P <.05). Conclusion: Enhanced LT generation from peripheral leukocytes is observed in patients with COPD, and the presence of specific IgE antibodies against inhalant allergens enhances LTC 4 generation, bronchial hyperresponsiveness, and the relationship between LTC 4 generation and airway obstruction.",
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AU - Mitsunobu, Fumihiro

AU - Mifune, Takashi

AU - Hosaki, Yasuhiro

AU - Ashida, Kozo

AU - Tsugeno, Hirofumi

AU - Okamoto, Makoto

AU - Takata, Shingo

AU - Tanizaki, Yoshiro

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N2 - Background: How leukotrienes (LTs) and IgE-mediated allergy reflect clinical features in patients with chronic obstructive pulmonary disease (COPD) remains unclear. Objective: Our goal was to determine whether LTB 4 and LTC 4 would correlate with airway obstruction and whether IgE-mediated allergy would influence the generation of LTs and bronchial hyperresponsiveness in patients with COPD. Methods: We measured the pulmonary function, methacholine bronchial hyperresponsiveness, and generation of LTB 4 and LTC 4 from peripheral leukocytes stimulated with calcium ionophore A23187 in relation to the presence of specific IgE antibodies against inhalant allergens. Results: The leukocytes of patients with COPD generated significantly more LTB 4 (with allergy, P <.001; without allergy, P <.001) and LTC 4 (with allergy, P <.001; without allergy, P <.01) than the leukocytes of the control subjects. LTC 4 production was significantly higher in the allergic COPD subjects than in the nonallergic COPD patients (P <.01), but the amount of LTB 4 generated was not significantly different. FEV 1 significantly correlated with the level of both LTB 4 (with allergy, r = -0.556, P = .0375; without allergy, r = -0.731, P = .0046) and LTC 4 (with allergy, r = -0.764, P = .0043; without allergy, r = -0.526, P = .0414) generation in COPD. The log 10 of the minimum dose of methacholine was significantly higher in COPD patients without allergy than in those with allergy (P <.05). Conclusion: Enhanced LT generation from peripheral leukocytes is observed in patients with COPD, and the presence of specific IgE antibodies against inhalant allergens enhances LTC 4 generation, bronchial hyperresponsiveness, and the relationship between LTC 4 generation and airway obstruction.

AB - Background: How leukotrienes (LTs) and IgE-mediated allergy reflect clinical features in patients with chronic obstructive pulmonary disease (COPD) remains unclear. Objective: Our goal was to determine whether LTB 4 and LTC 4 would correlate with airway obstruction and whether IgE-mediated allergy would influence the generation of LTs and bronchial hyperresponsiveness in patients with COPD. Methods: We measured the pulmonary function, methacholine bronchial hyperresponsiveness, and generation of LTB 4 and LTC 4 from peripheral leukocytes stimulated with calcium ionophore A23187 in relation to the presence of specific IgE antibodies against inhalant allergens. Results: The leukocytes of patients with COPD generated significantly more LTB 4 (with allergy, P <.001; without allergy, P <.001) and LTC 4 (with allergy, P <.001; without allergy, P <.01) than the leukocytes of the control subjects. LTC 4 production was significantly higher in the allergic COPD subjects than in the nonallergic COPD patients (P <.01), but the amount of LTB 4 generated was not significantly different. FEV 1 significantly correlated with the level of both LTB 4 (with allergy, r = -0.556, P = .0375; without allergy, r = -0.731, P = .0046) and LTC 4 (with allergy, r = -0.764, P = .0043; without allergy, r = -0.526, P = .0414) generation in COPD. The log 10 of the minimum dose of methacholine was significantly higher in COPD patients without allergy than in those with allergy (P <.05). Conclusion: Enhanced LT generation from peripheral leukocytes is observed in patients with COPD, and the presence of specific IgE antibodies against inhalant allergens enhances LTC 4 generation, bronchial hyperresponsiveness, and the relationship between LTC 4 generation and airway obstruction.

KW - Bronchial hyperresponsiveness

KW - COPD

KW - IgE-mediated allergy

KW - LTB

KW - LTC

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