Enhanced phosphorylation of PTEN in rat brain after transient middle cerebral artery occlusion

N. Omori, G. Jin, F. Li, W. R. Zhang, S. J. Wang, Y. Hamakawa, I. Nagano, Y. Manabe, M. Shoji, Koji Abe

Research output: Contribution to journalArticle

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Abstract

A phosphatase PTEN (phosphatase and tensin homologue deleted on chromosome 10) is a tumor suppressor gene that suppresses cell growth, inhibits cell migration, and induces apoptosis. Phosphorylated form of PTEN (p-PTEN) is a key survival factor relating PI3K-Akt pathway and their downstream effectors. A spatiotemporal profiles of PTEN and p-PTEN expression were immunohistochemically examined after 90 min of transient middle cerebral artery occlusion in rats. In the ischemic core, PTEN progressively decreased by 3 days, whereas a rapid but transient increase of p-PTEN was found with a peak at 1 h after the reperfusion. In contrast, in the ischemic penumbra, PTEN showed a minor change and a gradual but sustained p-PTEN expression was observed in the ischemic penumbra with a peak at 12 h. In addition, the balance of population among strongly, moderately, and weakly stained cells was different between the ischemic core and penumbra at their peak time points. These results suggest an important role of p-PTEN for cell survival after ischemia as an upstream regulator for PI3K-Akt.

Original languageEnglish
Pages (from-to)317-322
Number of pages6
JournalBrain Research
Volume954
Issue number2
DOIs
Publication statusPublished - Nov 8 2002

Fingerprint

Middle Cerebral Artery Infarction
Phosphatidylinositol 3-Kinases
Phosphorylation
PTEN Phosphohydrolase
Chromosomes, Human, Pair 10
Brain
Tumor Suppressor Genes
Phosphoric Monoester Hydrolases
Reperfusion
Cell Movement
Cell Survival
Ischemia
Apoptosis
Growth
Population
Tensins

Keywords

  • Akt
  • Apoptosis
  • MCAO
  • Phospho-PTEN
  • PI3K
  • PTEN

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Enhanced phosphorylation of PTEN in rat brain after transient middle cerebral artery occlusion. / Omori, N.; Jin, G.; Li, F.; Zhang, W. R.; Wang, S. J.; Hamakawa, Y.; Nagano, I.; Manabe, Y.; Shoji, M.; Abe, Koji.

In: Brain Research, Vol. 954, No. 2, 08.11.2002, p. 317-322.

Research output: Contribution to journalArticle

Omori, N, Jin, G, Li, F, Zhang, WR, Wang, SJ, Hamakawa, Y, Nagano, I, Manabe, Y, Shoji, M & Abe, K 2002, 'Enhanced phosphorylation of PTEN in rat brain after transient middle cerebral artery occlusion', Brain Research, vol. 954, no. 2, pp. 317-322. https://doi.org/10.1016/S0006-8993(02)03366-8
Omori, N. ; Jin, G. ; Li, F. ; Zhang, W. R. ; Wang, S. J. ; Hamakawa, Y. ; Nagano, I. ; Manabe, Y. ; Shoji, M. ; Abe, Koji. / Enhanced phosphorylation of PTEN in rat brain after transient middle cerebral artery occlusion. In: Brain Research. 2002 ; Vol. 954, No. 2. pp. 317-322.
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