Enhanced peripheral leukocyte leukotriene production and bronchial hyperresponsiveness in asthmatics

Fumihiro Mitsunobu, T. Mifune, Y. Hosaki, Kozo Ashida, H. Tsugeno, M. Okamoto, S. Harada, S. Takata, Y. Tanizaki, M. Harada

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Leukotrienes (LTs) are pro-inflammatory mediators that contribute to the pathophysiological features of asthma. The relationship between the amounts of LTB4 and LTC4 produced by the leukocytes of asthmatic patients on the one hand and immunoglobulin E (IgE)-mediated allergy, asthma exacerbations and bronchial hyperresponsiveness was studied. Leukocytes were obtained from peripheral blood drawn from 29 atopic and 27 nonatopic asthmatics during exacerbations and clinically controlled periods, as well as from 20 control individuals. The leukocytes were stimulated with calcium ionophore A23187 to induce LTB4 and LTC4 production. Allergy was assessed by means of specific serum IgE or by positive skin tests, whereas bronchial hyperresponsiveness was measured by methacholine challenge. The leukocytes of the asthmatics generated significantly more LTB4 (p4 (p4 than those of patients with nonatopic asthma (p4 was produced by leukocytes obtained during exacerbations, than by those obtained during clinically controlled periods (p4 generation by leukocytes and the degree of bronchial hyperresponsiveness to methacholine (r=-0.792, p4 production by leukocytes is associated with immunoglobulin E-mediated allergy and asthma exacerbations, and further that generation of leukotriene B4 is closely related to bronchial hyperresponsiveness in patients with asthma. (C) ERS Journals Ltd 2000.

Original languageEnglish
Pages (from-to)504-508
Number of pages5
JournalEuropean Respiratory Journal
Volume16
Issue number3
DOIs
Publication statusPublished - 2000

Fingerprint

Leukotrienes
Leukocytes
Leukotriene B4
Asthma
Immunoglobulin E
Leukotriene C4
Hypersensitivity
Methacholine Chloride
Calcium Ionophores
Calcimycin
Skin Tests
Serum

Keywords

  • Asthma attacks
  • Bronchial hyperresponsiveness
  • Immunoglobulin E-mediated allergy
  • Leukotriene B
  • Leukotriene C

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Enhanced peripheral leukocyte leukotriene production and bronchial hyperresponsiveness in asthmatics. / Mitsunobu, Fumihiro; Mifune, T.; Hosaki, Y.; Ashida, Kozo; Tsugeno, H.; Okamoto, M.; Harada, S.; Takata, S.; Tanizaki, Y.; Harada, M.

In: European Respiratory Journal, Vol. 16, No. 3, 2000, p. 504-508.

Research output: Contribution to journalArticle

Mitsunobu, Fumihiro ; Mifune, T. ; Hosaki, Y. ; Ashida, Kozo ; Tsugeno, H. ; Okamoto, M. ; Harada, S. ; Takata, S. ; Tanizaki, Y. ; Harada, M. / Enhanced peripheral leukocyte leukotriene production and bronchial hyperresponsiveness in asthmatics. In: European Respiratory Journal. 2000 ; Vol. 16, No. 3. pp. 504-508.
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