Enhanced Optical Collection of Micro- and Nanovesicles in the Presence of Gold Nanoparticles

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We describe a process for collecting micro- and nanovesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on a glass substrate that sandwiched a solution containing vesicles prepared using a phospholipid. The optical pressure generated at the surface of the vesicles pulled them into the center of the beam where they formed an aggregate on the glass surface. The vesicles prepared with a buffer solution were successfully collected via adsorption onto the glass surface, whereas the vesicles prepared with pure water exhibited no such tendency. The time required to collect a certain amount of vesicles was inversely proportional to their concentration. To enhance the collection efficiency, we added gold nanoparticles to the vesicle solution. The addition of gold nanoparticles into the solution reduced the collection time to one-tenth of that without it, and this was attributed to thermal mixing promoted by the heat generated by the absorption from the gold nanoparticles in the solution, as well as to an enhancement of light scattering induced by the gold nanoparticles. The optical collection of vesicles coupled with gold nanoparticles shows a promise for the collection of trace amounts of extracellular vesicles in biological fluids.

Original languageEnglish
Pages (from-to)2527-2531
Number of pages5
JournalACS Omega
Volume3
Issue number3
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Gold
Nanoparticles
Glass
Laser beams
Phospholipids
Substrates
Light scattering
Buffers
Adsorption
Fluids
Water
Hot Temperature

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

Cite this

Enhanced Optical Collection of Micro- and Nanovesicles in the Presence of Gold Nanoparticles. / Kuboi, Mai; Takeyasu, Nobuyuki; Kaneta, Takashi.

In: ACS Omega, Vol. 3, No. 3, 01.01.2018, p. 2527-2531.

Research output: Contribution to journalArticle

@article{61b9063012d64efc9392495806aa865f,
title = "Enhanced Optical Collection of Micro- and Nanovesicles in the Presence of Gold Nanoparticles",
abstract = "We describe a process for collecting micro- and nanovesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on a glass substrate that sandwiched a solution containing vesicles prepared using a phospholipid. The optical pressure generated at the surface of the vesicles pulled them into the center of the beam where they formed an aggregate on the glass surface. The vesicles prepared with a buffer solution were successfully collected via adsorption onto the glass surface, whereas the vesicles prepared with pure water exhibited no such tendency. The time required to collect a certain amount of vesicles was inversely proportional to their concentration. To enhance the collection efficiency, we added gold nanoparticles to the vesicle solution. The addition of gold nanoparticles into the solution reduced the collection time to one-tenth of that without it, and this was attributed to thermal mixing promoted by the heat generated by the absorption from the gold nanoparticles in the solution, as well as to an enhancement of light scattering induced by the gold nanoparticles. The optical collection of vesicles coupled with gold nanoparticles shows a promise for the collection of trace amounts of extracellular vesicles in biological fluids.",
author = "Mai Kuboi and Nobuyuki Takeyasu and Takashi Kaneta",
year = "2018",
month = "1",
day = "1",
doi = "10.1021/acsomega.8b00033",
language = "English",
volume = "3",
pages = "2527--2531",
journal = "ACS Omega",
issn = "2470-1343",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Enhanced Optical Collection of Micro- and Nanovesicles in the Presence of Gold Nanoparticles

AU - Kuboi, Mai

AU - Takeyasu, Nobuyuki

AU - Kaneta, Takashi

PY - 2018/1/1

Y1 - 2018/1/1

N2 - We describe a process for collecting micro- and nanovesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on a glass substrate that sandwiched a solution containing vesicles prepared using a phospholipid. The optical pressure generated at the surface of the vesicles pulled them into the center of the beam where they formed an aggregate on the glass surface. The vesicles prepared with a buffer solution were successfully collected via adsorption onto the glass surface, whereas the vesicles prepared with pure water exhibited no such tendency. The time required to collect a certain amount of vesicles was inversely proportional to their concentration. To enhance the collection efficiency, we added gold nanoparticles to the vesicle solution. The addition of gold nanoparticles into the solution reduced the collection time to one-tenth of that without it, and this was attributed to thermal mixing promoted by the heat generated by the absorption from the gold nanoparticles in the solution, as well as to an enhancement of light scattering induced by the gold nanoparticles. The optical collection of vesicles coupled with gold nanoparticles shows a promise for the collection of trace amounts of extracellular vesicles in biological fluids.

AB - We describe a process for collecting micro- and nanovesicles on a glass substrate using the optical pressure of a laser beam. The laser beam was focused on a glass substrate that sandwiched a solution containing vesicles prepared using a phospholipid. The optical pressure generated at the surface of the vesicles pulled them into the center of the beam where they formed an aggregate on the glass surface. The vesicles prepared with a buffer solution were successfully collected via adsorption onto the glass surface, whereas the vesicles prepared with pure water exhibited no such tendency. The time required to collect a certain amount of vesicles was inversely proportional to their concentration. To enhance the collection efficiency, we added gold nanoparticles to the vesicle solution. The addition of gold nanoparticles into the solution reduced the collection time to one-tenth of that without it, and this was attributed to thermal mixing promoted by the heat generated by the absorption from the gold nanoparticles in the solution, as well as to an enhancement of light scattering induced by the gold nanoparticles. The optical collection of vesicles coupled with gold nanoparticles shows a promise for the collection of trace amounts of extracellular vesicles in biological fluids.

UR - http://www.scopus.com/inward/record.url?scp=85042874705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85042874705&partnerID=8YFLogxK

U2 - 10.1021/acsomega.8b00033

DO - 10.1021/acsomega.8b00033

M3 - Article

VL - 3

SP - 2527

EP - 2531

JO - ACS Omega

JF - ACS Omega

SN - 2470-1343

IS - 3

ER -