Enhanced mitogen-induced proliferation of rat splenocytes by low-dose whole-body X-irradiation.

K. Ishii, Kiyonori Yamaoka, Y. Hosoi, T. Ono, K. Sakamoto

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

To elucidate the stimulative effect on the immune system, we studied the effect of acute low-dose whole-body X-irradiation on the mitogen-induced proliferative responses of rat splenocytes and thymocytes. Concanavalin A (Con A)-induced mitogen response of rat splenocytes 4 hours after 5 cGy irradiation was significantly higher (by 80%, p <0.02) than that in sham-irradiated controls; whereas that of rat thymocytes did not show change after irradiation of 1-10 cGy. In the rats X-irradiated with doses greater than 25 cGy, Con A-induced mitogen responses of splenocytes and thymocytes were reduced. In a way similar to the Con A-induced mitogen response of splenocytes, the mitogen response of splenocytes induced by phytohemagglutinin (PHA) or lipopolysaccharide (LPS) was significantly increased by X-irradiation with 5 cGy (by 50%, p <0.02). The enhancement of mitogen response of splenocytes was observed within a few hours after irradiation, being a temporary effect. After 5 cGy irradiation the level of leukotriene B4 (LTB4) in the supernatant fraction of the splenocyte suspension showed a significant increase (by 40%, p <0.05).

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalPhysiological Chemistry and Physics and Medical NMR
Volume27
Issue number1
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Whole-Body Irradiation
Mitogens
Dosimetry
Rats
Irradiation
Thymocytes
Concanavalin A
Leukotriene B4
Immune system
Phytohemagglutinins
Lipopolysaccharides
Immune System
Suspensions

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Physiology
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Enhanced mitogen-induced proliferation of rat splenocytes by low-dose whole-body X-irradiation. / Ishii, K.; Yamaoka, Kiyonori; Hosoi, Y.; Ono, T.; Sakamoto, K.

In: Physiological Chemistry and Physics and Medical NMR, Vol. 27, No. 1, 1995, p. 17-23.

Research output: Contribution to journalArticle

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