TY - JOUR
T1 - Enhanced intracellular delivery using arginine-rich peptides by the addition of penetration accelerating sequences (Pas)
AU - Takayama, Kentaro
AU - Nakase, Ikuhiko
AU - Michiue, Hiroyuki
AU - Takeuchi, Toshihide
AU - Tomizawa, Kazuhito
AU - Matsui, Hideki
AU - Futaki, Shiroh
N1 - Funding Information:
This work was supported in part by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan and from the Ministry of Health, Labour and Welfare of Japan. K. T. is grateful for a JSPS Research Fellowship for Young Scientists.
PY - 2009/9/1
Y1 - 2009/9/1
N2 - Cell penetrating peptides (CPPs), including arginine-rich peptides, are attractive tools for the intracellular delivery of various bioactive molecules with a low membrane permeability. We showed that the accelerated intracellular delivery of arginine-rich peptides was achieved by the addition of a short peptide segment (penetration accelerating sequence, Pas) to arginine-rich CPPs. The cytosolic release of the Pas-attached arginine-rich CPPs was observed within 5 min after the treatment of the cells with the peptides even in the presence of serum. Effectiveness of the Pas segment in the intracellular delivery of bioactive peptides using arginine-rich CPPs was exemplified through the enhanced growth inhibition activity of the malignant glioma cells by a retro-inverso peptide derived from the p53 C-terminal 22-amino-acid segment (positions 361-382).
AB - Cell penetrating peptides (CPPs), including arginine-rich peptides, are attractive tools for the intracellular delivery of various bioactive molecules with a low membrane permeability. We showed that the accelerated intracellular delivery of arginine-rich peptides was achieved by the addition of a short peptide segment (penetration accelerating sequence, Pas) to arginine-rich CPPs. The cytosolic release of the Pas-attached arginine-rich CPPs was observed within 5 min after the treatment of the cells with the peptides even in the presence of serum. Effectiveness of the Pas segment in the intracellular delivery of bioactive peptides using arginine-rich CPPs was exemplified through the enhanced growth inhibition activity of the malignant glioma cells by a retro-inverso peptide derived from the p53 C-terminal 22-amino-acid segment (positions 361-382).
KW - Arginine-rich peptide
KW - Cell penetrating peptide
KW - Intracellular delivery
KW - Retro-inverso peptide
KW - p53 C-terminal segment
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U2 - 10.1016/j.jconrel.2009.05.019
DO - 10.1016/j.jconrel.2009.05.019
M3 - Article
C2 - 19465072
AN - SCOPUS:67749115965
VL - 138
SP - 128
EP - 133
JO - Journal of Controlled Release
JF - Journal of Controlled Release
SN - 0168-3659
IS - 2
ER -