Abstract
Cell penetrating peptides (CPPs), including arginine-rich peptides, are attractive tools for the intracellular delivery of various bioactive molecules with a low membrane permeability. We showed that the accelerated intracellular delivery of arginine-rich peptides was achieved by the addition of a short peptide segment (penetration accelerating sequence, Pas) to arginine-rich CPPs. The cytosolic release of the Pas-attached arginine-rich CPPs was observed within 5 min after the treatment of the cells with the peptides even in the presence of serum. Effectiveness of the Pas segment in the intracellular delivery of bioactive peptides using arginine-rich CPPs was exemplified through the enhanced growth inhibition activity of the malignant glioma cells by a retro-inverso peptide derived from the p53 C-terminal 22-amino-acid segment (positions 361-382).
| Original language | English |
|---|---|
| Pages (from-to) | 128-133 |
| Number of pages | 6 |
| Journal | Journal of Controlled Release |
| Volume | 138 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sep 1 2009 |
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Keywords
- Arginine-rich peptide
- Cell penetrating peptide
- Intracellular delivery
- p53 C-terminal segment
- Retro-inverso peptide
ASJC Scopus subject areas
- Pharmaceutical Science
Cite this
Enhanced intracellular delivery using arginine-rich peptides by the addition of penetration accelerating sequences (Pas). / Takayama, Kentaro; Nakase, Ikuhiko; Michiue, Hiroyuki; Takeuchi, Toshihide; Tomizawa, Kazuhito; Matsui, Hideki; Futaki, Shiroh.
In: Journal of Controlled Release, Vol. 138, No. 2, 01.09.2009, p. 128-133.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced intracellular delivery using arginine-rich peptides by the addition of penetration accelerating sequences (Pas)
AU - Takayama, Kentaro
AU - Nakase, Ikuhiko
AU - Michiue, Hiroyuki
AU - Takeuchi, Toshihide
AU - Tomizawa, Kazuhito
AU - Matsui, Hideki
AU - Futaki, Shiroh
PY - 2009/9/1
Y1 - 2009/9/1
N2 - Cell penetrating peptides (CPPs), including arginine-rich peptides, are attractive tools for the intracellular delivery of various bioactive molecules with a low membrane permeability. We showed that the accelerated intracellular delivery of arginine-rich peptides was achieved by the addition of a short peptide segment (penetration accelerating sequence, Pas) to arginine-rich CPPs. The cytosolic release of the Pas-attached arginine-rich CPPs was observed within 5 min after the treatment of the cells with the peptides even in the presence of serum. Effectiveness of the Pas segment in the intracellular delivery of bioactive peptides using arginine-rich CPPs was exemplified through the enhanced growth inhibition activity of the malignant glioma cells by a retro-inverso peptide derived from the p53 C-terminal 22-amino-acid segment (positions 361-382).
AB - Cell penetrating peptides (CPPs), including arginine-rich peptides, are attractive tools for the intracellular delivery of various bioactive molecules with a low membrane permeability. We showed that the accelerated intracellular delivery of arginine-rich peptides was achieved by the addition of a short peptide segment (penetration accelerating sequence, Pas) to arginine-rich CPPs. The cytosolic release of the Pas-attached arginine-rich CPPs was observed within 5 min after the treatment of the cells with the peptides even in the presence of serum. Effectiveness of the Pas segment in the intracellular delivery of bioactive peptides using arginine-rich CPPs was exemplified through the enhanced growth inhibition activity of the malignant glioma cells by a retro-inverso peptide derived from the p53 C-terminal 22-amino-acid segment (positions 361-382).
KW - Arginine-rich peptide
KW - Cell penetrating peptide
KW - Intracellular delivery
KW - p53 C-terminal segment
KW - Retro-inverso peptide
UR - http://www.scopus.com/inward/record.url?scp=67749115965&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=67749115965&partnerID=8YFLogxK
U2 - 10.1016/j.jconrel.2009.05.019
DO - 10.1016/j.jconrel.2009.05.019
M3 - Article
C2 - 19465072
AN - SCOPUS:67749115965
VL - 138
SP - 128
EP - 133
JO - Journal of Controlled Release
JF - Journal of Controlled Release
SN - 0168-3659
IS - 2
ER -