Enhanced intracellular delivery using arginine-rich peptides by the addition of penetration accelerating sequences (Pas)

Kentaro Takayama; Ikuhiko Nakase; Hiroyuki Michiue; Toshihide Takeuchi; Kazuhito Tomizawa; Hideki Matsui; Shiroh Futaki

doi:10.1016/j.jconrel.2009.05.019

Enhanced intracellular delivery using arginine-rich peptides by the addition of penetration accelerating sequences (Pas)

Kentaro Takayama, Ikuhiko Nakase, Hiroyuki Michiue, Toshihide Takeuchi, Kazuhito Tomizawa, Hideki Matsui, Shiroh Futaki

Neutron Therapy Research Center

Research output: Contribution to journal › Article › peer-review

74 Citations (Scopus)

Abstract

Cell penetrating peptides (CPPs), including arginine-rich peptides, are attractive tools for the intracellular delivery of various bioactive molecules with a low membrane permeability. We showed that the accelerated intracellular delivery of arginine-rich peptides was achieved by the addition of a short peptide segment (penetration accelerating sequence, Pas) to arginine-rich CPPs. The cytosolic release of the Pas-attached arginine-rich CPPs was observed within 5 min after the treatment of the cells with the peptides even in the presence of serum. Effectiveness of the Pas segment in the intracellular delivery of bioactive peptides using arginine-rich CPPs was exemplified through the enhanced growth inhibition activity of the malignant glioma cells by a retro-inverso peptide derived from the p53 C-terminal 22-amino-acid segment (positions 361-382).

Original language	English
Pages (from-to)	128-133
Number of pages	6
Journal	Journal of Controlled Release
Volume	138
Issue number	2
DOIs	https://doi.org/10.1016/j.jconrel.2009.05.019
Publication status	Published - Sep 1 2009

Keywords

Arginine-rich peptide
Cell penetrating peptide
Intracellular delivery
Retro-inverso peptide
p53 C-terminal segment

ASJC Scopus subject areas

Pharmaceutical Science

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Enhanced intracellular delivery using arginine-rich peptides by the addition of penetration accelerating sequences (Pas). / Takayama, Kentaro; Nakase, Ikuhiko; Michiue, Hiroyuki; Takeuchi, Toshihide; Tomizawa, Kazuhito; Matsui, Hideki; Futaki, Shiroh.

In: Journal of Controlled Release, Vol. 138, No. 2, 01.09.2009, p. 128-133.

Research output: Contribution to journal › Article › peer-review

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AU - Tomizawa, Kazuhito

AU - Matsui, Hideki

AU - Futaki, Shiroh

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