Abstract
The etiology of amyotrophic lateral sclerosis (ALS) remains unknown although an existence of neurotoxic substances in cerebrospinal fluid (CSF) from ALS patients have been postulated. In order to investigate a possible effect of CSF from ALS patients on cellular signaling in spinal neurons, we compared Fos-like immunoreactivity (Fos-LI) in organotypic cultures of rat lumbar spinal cord after addition of CSF from ALS patients or another neurologic disease. Fos-LI was normally present predominantly in dorsal horn neurons, whereas only a few ventral horn neurons were positive for Fos-LI. The number of Fos-LI positive neurons significantly increased in dorsal horn with addition of CSF from ALS patients as well as glutamate at 100 μM. However, the increase was not observed with addition of CSF from other neurologic diseases. The increase in Fos-LI positive neurons in dorsal horn was reversed by a further supplement of MK801, an N-methyl-D-aspartate (NMDA) receptor antagonist, but not of CNQX, an α-amino-3-hydroxy-5-methyl-4- isoxazole propionic acid (AMPA)/kainate antagonist. These results indicate that there may be substances in CSF from ALS patients that stimulate Fos expression in certain populations of spinal neurons via the NMDA receptors.
| Original language | English |
|---|---|
| Pages (from-to) | 309-312 |
| Number of pages | 4 |
| Journal | Neurological Research |
| Volume | 21 |
| Issue number | 3 |
| Publication status | Published - 1999 |
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Keywords
- Amyotrophic lateral sclerosis
- C-fos expression
- Cerebrospinal fluid
- Slice culture
- Spinal cord
ASJC Scopus subject areas
- Clinical Neurology
- Neuroscience(all)
Cite this
Enhanced Fos expression in rat lumbar spinal cord cultured with cerebrospinal fluid from patients with amyotrophic lateral sclerosis. / Manabe, Yasuhiro; Kashihara, Kenichi; Shiro, Yoshihiko; Shohmori, Toshikiyo; Abe, Koji.
In: Neurological Research, Vol. 21, No. 3, 1999, p. 309-312.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced Fos expression in rat lumbar spinal cord cultured with cerebrospinal fluid from patients with amyotrophic lateral sclerosis
AU - Manabe, Yasuhiro
AU - Kashihara, Kenichi
AU - Shiro, Yoshihiko
AU - Shohmori, Toshikiyo
AU - Abe, Koji
PY - 1999
Y1 - 1999
N2 - The etiology of amyotrophic lateral sclerosis (ALS) remains unknown although an existence of neurotoxic substances in cerebrospinal fluid (CSF) from ALS patients have been postulated. In order to investigate a possible effect of CSF from ALS patients on cellular signaling in spinal neurons, we compared Fos-like immunoreactivity (Fos-LI) in organotypic cultures of rat lumbar spinal cord after addition of CSF from ALS patients or another neurologic disease. Fos-LI was normally present predominantly in dorsal horn neurons, whereas only a few ventral horn neurons were positive for Fos-LI. The number of Fos-LI positive neurons significantly increased in dorsal horn with addition of CSF from ALS patients as well as glutamate at 100 μM. However, the increase was not observed with addition of CSF from other neurologic diseases. The increase in Fos-LI positive neurons in dorsal horn was reversed by a further supplement of MK801, an N-methyl-D-aspartate (NMDA) receptor antagonist, but not of CNQX, an α-amino-3-hydroxy-5-methyl-4- isoxazole propionic acid (AMPA)/kainate antagonist. These results indicate that there may be substances in CSF from ALS patients that stimulate Fos expression in certain populations of spinal neurons via the NMDA receptors.
AB - The etiology of amyotrophic lateral sclerosis (ALS) remains unknown although an existence of neurotoxic substances in cerebrospinal fluid (CSF) from ALS patients have been postulated. In order to investigate a possible effect of CSF from ALS patients on cellular signaling in spinal neurons, we compared Fos-like immunoreactivity (Fos-LI) in organotypic cultures of rat lumbar spinal cord after addition of CSF from ALS patients or another neurologic disease. Fos-LI was normally present predominantly in dorsal horn neurons, whereas only a few ventral horn neurons were positive for Fos-LI. The number of Fos-LI positive neurons significantly increased in dorsal horn with addition of CSF from ALS patients as well as glutamate at 100 μM. However, the increase was not observed with addition of CSF from other neurologic diseases. The increase in Fos-LI positive neurons in dorsal horn was reversed by a further supplement of MK801, an N-methyl-D-aspartate (NMDA) receptor antagonist, but not of CNQX, an α-amino-3-hydroxy-5-methyl-4- isoxazole propionic acid (AMPA)/kainate antagonist. These results indicate that there may be substances in CSF from ALS patients that stimulate Fos expression in certain populations of spinal neurons via the NMDA receptors.
KW - Amyotrophic lateral sclerosis
KW - C-fos expression
KW - Cerebrospinal fluid
KW - Slice culture
KW - Spinal cord
UR - http://www.scopus.com/inward/record.url?scp=0032913118&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032913118&partnerID=8YFLogxK
M3 - Article
VL - 21
SP - 309
EP - 312
JO - Neurological Research
JF - Neurological Research
SN - 0161-6412
IS - 3
ER -