Enhanced expression of CXCL13 in human helicobacter pylori-associated gastritis

Yujiro Nakashima, Hajime Isomoto, Kayoko Matsushima, Akira Yoshida, Toshiyuki Nakayama, Masaaki Nakayama, Junzo Hisatsune, Tatsuki Ichikawa, Fuminao Takeshima, Tomayoshi Hayashi, Kazuhiko Nakao, Toshiya Hirayama, Shigeru Kohno

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background and Aims: Chemokine CXC ligand 13 (CXCL13) and CXC receptor type 5 (CXCR5) are constitutively expressed in tertiary lymphoid follicles where the CXCL13/CXCR5 system regulates B lymphocytes homing. In this study, we sought to examine CXCL13 expression in the H. pylori-infected and -uninfected gastric mucosa and to elucidate the implication in the pathogenesis of HAG in humans. Methods: Using endoscopic biopsies taken from the gastric antrum of 29 subjects infected with Helicobacter pylori and 22 uninfected subjects, mucosal CXCL13 mRNA and protein levels were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: The CXCL13 expression levels were significantly more elevated in H. pylori-positive patients than uninfected ones. The CXCL13 expression levels correlated with the degree of chronic gastritis and bacterial colonization. Immunohistochemistry and in vitro infection assay showed that CXCL13 was not produced by the gastric epithelium, but the α-smooth muscle antigen expressing mesenchymal cells were the possible source of CXCL13 within H. pylori-infected gastric mucosa. CXCR5 immunostaining was seen in the CD20-positive lymphoid aggregates. Conclusions: The enhanced induction of CXCL13 may be involved in the pathogenesis of H. pylori-associated gastritis.

Original languageEnglish
Pages (from-to)2887-2894
Number of pages8
JournalDigestive Diseases and Sciences
Volume56
Issue number10
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Fingerprint

Gastritis
Helicobacter pylori
Ligands
Gastric Mucosa
Pyloric Antrum
CXC Chemokines
Smooth Muscle
Real-Time Polymerase Chain Reaction
Stomach
B-Lymphocytes
Epithelium
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Biopsy
Antigens
Messenger RNA
Infection

Keywords

  • α-Smooth muscle antigen
  • Chemokine CXC ligand 13
  • Chronic inflammation
  • CXC receptor type 5
  • Helicobacter pylori

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Nakashima, Y., Isomoto, H., Matsushima, K., Yoshida, A., Nakayama, T., Nakayama, M., ... Kohno, S. (2011). Enhanced expression of CXCL13 in human helicobacter pylori-associated gastritis. Digestive Diseases and Sciences, 56(10), 2887-2894. https://doi.org/10.1007/s10620-011-1717-8

Enhanced expression of CXCL13 in human helicobacter pylori-associated gastritis. / Nakashima, Yujiro; Isomoto, Hajime; Matsushima, Kayoko; Yoshida, Akira; Nakayama, Toshiyuki; Nakayama, Masaaki; Hisatsune, Junzo; Ichikawa, Tatsuki; Takeshima, Fuminao; Hayashi, Tomayoshi; Nakao, Kazuhiko; Hirayama, Toshiya; Kohno, Shigeru.

In: Digestive Diseases and Sciences, Vol. 56, No. 10, 10.2011, p. 2887-2894.

Research output: Contribution to journalArticle

Nakashima, Y, Isomoto, H, Matsushima, K, Yoshida, A, Nakayama, T, Nakayama, M, Hisatsune, J, Ichikawa, T, Takeshima, F, Hayashi, T, Nakao, K, Hirayama, T & Kohno, S 2011, 'Enhanced expression of CXCL13 in human helicobacter pylori-associated gastritis', Digestive Diseases and Sciences, vol. 56, no. 10, pp. 2887-2894. https://doi.org/10.1007/s10620-011-1717-8
Nakashima, Yujiro ; Isomoto, Hajime ; Matsushima, Kayoko ; Yoshida, Akira ; Nakayama, Toshiyuki ; Nakayama, Masaaki ; Hisatsune, Junzo ; Ichikawa, Tatsuki ; Takeshima, Fuminao ; Hayashi, Tomayoshi ; Nakao, Kazuhiko ; Hirayama, Toshiya ; Kohno, Shigeru. / Enhanced expression of CXCL13 in human helicobacter pylori-associated gastritis. In: Digestive Diseases and Sciences. 2011 ; Vol. 56, No. 10. pp. 2887-2894.
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abstract = "Background and Aims: Chemokine CXC ligand 13 (CXCL13) and CXC receptor type 5 (CXCR5) are constitutively expressed in tertiary lymphoid follicles where the CXCL13/CXCR5 system regulates B lymphocytes homing. In this study, we sought to examine CXCL13 expression in the H. pylori-infected and -uninfected gastric mucosa and to elucidate the implication in the pathogenesis of HAG in humans. Methods: Using endoscopic biopsies taken from the gastric antrum of 29 subjects infected with Helicobacter pylori and 22 uninfected subjects, mucosal CXCL13 mRNA and protein levels were measured by real-time polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. Results: The CXCL13 expression levels were significantly more elevated in H. pylori-positive patients than uninfected ones. The CXCL13 expression levels correlated with the degree of chronic gastritis and bacterial colonization. Immunohistochemistry and in vitro infection assay showed that CXCL13 was not produced by the gastric epithelium, but the α-smooth muscle antigen expressing mesenchymal cells were the possible source of CXCL13 within H. pylori-infected gastric mucosa. CXCR5 immunostaining was seen in the CD20-positive lymphoid aggregates. Conclusions: The enhanced induction of CXCL13 may be involved in the pathogenesis of H. pylori-associated gastritis.",
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AU - Hisatsune, Junzo

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