Abstract
Recent studies have shown that bone morphogenetic proteins (BMPs), particularly BMP-7, have an inhibitory role in the development of various renal diseases. We previously reported antagonistic effects of BMPs on renal mesangial cell proliferation induced by aldosterone (Aldo) in vitro. In the present study, we investigated in vivo roles of BMPs in Aldo-induced renal glomerular injury. BALB/c mice aged 6 weeks were treated with Aldo injection (5 g per day, intraperitoneally) and/or oral administration of high-salt (2%) water for 9 weeks. Systemic blood pressure, body weight, kidney weight and daily proteinuria were not significantly changed by Aldo and/or high-salt treatment. However, renal histological examination revealed increases in glomerular cellularity and glomerular diameter in the groups treated with Aldo injection and high-salt administration. Immunohistochemistry demonstrated expression of BMP-4 and-7 in the glomerular mesangial region. Aldo causes renal glomerular damage by stimulating mesangial cell proliferation and increasing extracellular matrix via the mineralocorticoid receptor (MR). MR messenger RNA (mRNA) expression in the renal cortex was transiently increased by 3-week treatment with Aldo and high-salt intake, but was decreased by 9-week treatment. Furthermore, the expression levels of BMP-4 and-7 mRNA were enhanced in the renal cortex treated with Aldo and high-salt administration. These findings suggest that the renal BMP system is activated by Aldo under the condition of high-salt exposure, which may have a key role in antagonizing glomerular damage in vivo.
| Original language | English |
|---|---|
| Pages (from-to) | 312-317 |
| Number of pages | 6 |
| Journal | Hypertension Research |
| Volume | 35 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2012 |
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Keywords
- aldosterone
- bone morphogenetic protein (BMP)
- glomerulus
- kidney
- salt-sensitive hypertension
ASJC Scopus subject areas
- Internal Medicine
- Physiology
- Cardiology and Cardiovascular Medicine
Cite this
Enhanced expression of bone morphogenetic protein system in aldosterone-treated mouse kidneys. / Suzuki, Jiro; Otsuka, Fumio; Matsumoto, Yoshinori; Inagaki, Kenichi; Miyoshi, Tomoko; Takeda, Masaya; Tsukamoto, Naoko; Nakamura, Eri; Ogura, Kanako; Makino, Hirofumi.
In: Hypertension Research, Vol. 35, No. 3, 03.2012, p. 312-317.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Enhanced expression of bone morphogenetic protein system in aldosterone-treated mouse kidneys
AU - Suzuki, Jiro
AU - Otsuka, Fumio
AU - Matsumoto, Yoshinori
AU - Inagaki, Kenichi
AU - Miyoshi, Tomoko
AU - Takeda, Masaya
AU - Tsukamoto, Naoko
AU - Nakamura, Eri
AU - Ogura, Kanako
AU - Makino, Hirofumi
PY - 2012/3
Y1 - 2012/3
N2 - Recent studies have shown that bone morphogenetic proteins (BMPs), particularly BMP-7, have an inhibitory role in the development of various renal diseases. We previously reported antagonistic effects of BMPs on renal mesangial cell proliferation induced by aldosterone (Aldo) in vitro. In the present study, we investigated in vivo roles of BMPs in Aldo-induced renal glomerular injury. BALB/c mice aged 6 weeks were treated with Aldo injection (5 g per day, intraperitoneally) and/or oral administration of high-salt (2%) water for 9 weeks. Systemic blood pressure, body weight, kidney weight and daily proteinuria were not significantly changed by Aldo and/or high-salt treatment. However, renal histological examination revealed increases in glomerular cellularity and glomerular diameter in the groups treated with Aldo injection and high-salt administration. Immunohistochemistry demonstrated expression of BMP-4 and-7 in the glomerular mesangial region. Aldo causes renal glomerular damage by stimulating mesangial cell proliferation and increasing extracellular matrix via the mineralocorticoid receptor (MR). MR messenger RNA (mRNA) expression in the renal cortex was transiently increased by 3-week treatment with Aldo and high-salt intake, but was decreased by 9-week treatment. Furthermore, the expression levels of BMP-4 and-7 mRNA were enhanced in the renal cortex treated with Aldo and high-salt administration. These findings suggest that the renal BMP system is activated by Aldo under the condition of high-salt exposure, which may have a key role in antagonizing glomerular damage in vivo.
AB - Recent studies have shown that bone morphogenetic proteins (BMPs), particularly BMP-7, have an inhibitory role in the development of various renal diseases. We previously reported antagonistic effects of BMPs on renal mesangial cell proliferation induced by aldosterone (Aldo) in vitro. In the present study, we investigated in vivo roles of BMPs in Aldo-induced renal glomerular injury. BALB/c mice aged 6 weeks were treated with Aldo injection (5 g per day, intraperitoneally) and/or oral administration of high-salt (2%) water for 9 weeks. Systemic blood pressure, body weight, kidney weight and daily proteinuria were not significantly changed by Aldo and/or high-salt treatment. However, renal histological examination revealed increases in glomerular cellularity and glomerular diameter in the groups treated with Aldo injection and high-salt administration. Immunohistochemistry demonstrated expression of BMP-4 and-7 in the glomerular mesangial region. Aldo causes renal glomerular damage by stimulating mesangial cell proliferation and increasing extracellular matrix via the mineralocorticoid receptor (MR). MR messenger RNA (mRNA) expression in the renal cortex was transiently increased by 3-week treatment with Aldo and high-salt intake, but was decreased by 9-week treatment. Furthermore, the expression levels of BMP-4 and-7 mRNA were enhanced in the renal cortex treated with Aldo and high-salt administration. These findings suggest that the renal BMP system is activated by Aldo under the condition of high-salt exposure, which may have a key role in antagonizing glomerular damage in vivo.
KW - aldosterone
KW - bone morphogenetic protein (BMP)
KW - glomerulus
KW - kidney
KW - salt-sensitive hypertension
UR - http://www.scopus.com/inward/record.url?scp=84857982783&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84857982783&partnerID=8YFLogxK
U2 - 10.1038/hr.2011.186
DO - 10.1038/hr.2011.186
M3 - Article
C2 - 22072104
AN - SCOPUS:84857982783
VL - 35
SP - 312
EP - 317
JO - Hypertension Research
JF - Hypertension Research
SN - 0916-9636
IS - 3
ER -