Enhanced early osteogenic differentiation by silicon-substituted hydroxyapatite ceramics fabricated via ultrasonic spray pyrolysis route

Michiyo Honda, Koichi Kikushima, Yusuke Kawanobe, Toshiisa Konishi, Minori Mizumoto, Mamoru Aizawa

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The influence of silicon-substituted hydroxyapatite (Si-HAp) on osteogenic differentiation was assessed by biological analysis. Si-HAp was prepared by ultrasonic spray pyrolysis (USSP) technique using various amounts of Si (0, 0.8, and 1.6 mass%). Chemical analysis revealed that Si was incorporated into the hydroxyapatite (HAp) lattice with no other crystalline phase and which caused the change of crystal structure. Biological analyses showed that the Si contents affected the cell proliferation and morphology, suggesting that there is an optimal Si content for cell culture. As for differentiation, alkaline phosphatase activity and osteocalcin production of Si-HAp were higher than those of HAp. Gene expression profiles also revealed that substitution of Si (0.8 mass%) up-regulated the expression levels of osteocalcin and especially Runx2, a master gene for osteoblast development. These results suggest that incorporating Si into the HAp lattice may enhance the bioactivity, particularly during early osteoblast development.

Original languageEnglish
Pages (from-to)2923-2932
Number of pages10
JournalJournal of Materials Science: Materials in Medicine
Volume23
Issue number12
DOIs
Publication statusPublished - Dec 2012
Externally publishedYes

Fingerprint

Spray pyrolysis
Ceramics
Silicon
Durapatite
Hydroxyapatite
Ultrasonics
Osteocalcin
Osteoblasts
Phosphatases
Cell proliferation
Bioactivity
Transcriptome
Cell culture
Gene expression
Crystal lattices
Alkaline Phosphatase
Substitution reactions
Cell Culture Techniques
Genes
Crystal structure

ASJC Scopus subject areas

  • Biophysics
  • Biomaterials
  • Bioengineering
  • Biomedical Engineering

Cite this

Enhanced early osteogenic differentiation by silicon-substituted hydroxyapatite ceramics fabricated via ultrasonic spray pyrolysis route. / Honda, Michiyo; Kikushima, Koichi; Kawanobe, Yusuke; Konishi, Toshiisa; Mizumoto, Minori; Aizawa, Mamoru.

In: Journal of Materials Science: Materials in Medicine, Vol. 23, No. 12, 12.2012, p. 2923-2932.

Research output: Contribution to journalArticle

Honda, Michiyo ; Kikushima, Koichi ; Kawanobe, Yusuke ; Konishi, Toshiisa ; Mizumoto, Minori ; Aizawa, Mamoru. / Enhanced early osteogenic differentiation by silicon-substituted hydroxyapatite ceramics fabricated via ultrasonic spray pyrolysis route. In: Journal of Materials Science: Materials in Medicine. 2012 ; Vol. 23, No. 12. pp. 2923-2932.
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