Enhanced cellular uptake of lactosomes using cell-penetrating peptides

Akiya Akahoshi, Eiji Matsuura, Eiichi Ozeki, Hayato Matsui, Kazunori Watanabe, Takashi Ohtsuki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Polymeric micelles that are composed of synthetic polymers are generally size controllable and can be easily modified for various applications. Lactosomes (A3B-type) are biodegradable polymeric micelles composed of an amphipathic polymer, including three poly(sarcosine) blocks and a poly(l-lactic acid) block. Lactosomes accumulate in tumors in vivo through the enhanced permeability and retention (EPR) effect, even on frequently administering them. However, lactosomes cannot be efficiently internalized by cells. To improve cellular uptake of lactosomes, cell-penetrating peptide (CPP)-modified lactosomes were prepared. Seven CPPs (including EB1 and Pep1) were used, and most of them improved the cellular uptake efficiency of lactosomes. In particular, EB1- and Pep1-modified lactosomes were efficiently internalized by cells. In addition, by using CPP-modified and photosensitizer-loaded lactosomes, we demonstrated the photoinduced killing of mammalian cells, including human cancer cells. Accumulation of the EB1-modified lactosomes in NCI-N87 tumors was shown by in vivo imaging. Thus, this study demonstrated that the CPP-modified lactosome is a promising drug carrier.

Original languageEnglish
Pages (from-to)245-252
Number of pages8
JournalScience and Technology of Advanced Materials
Volume17
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Cell-Penetrating Peptides
Peptides
Micelles
Tumors
Polymers
Cells
Sarcosine
Drug Carriers
Photosensitizing Agents
Photosensitizers
Lactic acid
Lactic Acid
Imaging techniques

Keywords

  • Cell penetrating peptide
  • drug delivery
  • lactosome
  • photosensitizer
  • polymeric micelle

ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Enhanced cellular uptake of lactosomes using cell-penetrating peptides. / Akahoshi, Akiya; Matsuura, Eiji; Ozeki, Eiichi; Matsui, Hayato; Watanabe, Kazunori; Ohtsuki, Takashi.

In: Science and Technology of Advanced Materials, Vol. 17, No. 1, 01.01.2016, p. 245-252.

Research output: Contribution to journalArticle

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