Enhanced angiogenesis in bFGF-containing scaffold promoted viability of enclosed hepatocytes and maintained hepatospecific glycogen storage capacity

Takayuki Takei, Hiroyuki Ijima, Shinji Sakai, Tsutomu Ono, Koei Kawakami

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We fabricated a scaffold of poly (lactic acid) and acidic gelatin, a sustained-release carrier of basic fibroblast growth factor (bFGF), which is a positive regulator of angiogenesis. After 1 week of implantation into mesentery of rats, blood vessels induced in the bFGF-containing scaffolds were almost double those in the bFGF-free scaffolds. Further, histological examination of hepatocyte-immobilizing scaffolds retrieved after 1 week of implantation into the mesentery of the rats that received a 70% hepatectomy revealed that the bFGF-containing scaffolds were more efficient for immobilized cell survival than the bFGF-free scaffolds. As well, hepatocytes in the bFGF-containing scaffolds kept their hepatospecific glycogen storage capacity.

Original languageEnglish
Pages (from-to)913-917
Number of pages5
JournalJournal of Chemical Engineering of Japan
Volume38
Issue number11
DOIs
Publication statusPublished - Nov 20 2005
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 2
Fibroblasts
Glycogen
Scaffolds
Rats
Blood vessels
Gelatin
Lactic acid
Hepatocytes
Intercellular Signaling Peptides and Proteins
Cells

Keywords

  • Angiogenesis
  • bFGF
  • Cell transplantation
  • Hepatocyte
  • Scaffold

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Enhanced angiogenesis in bFGF-containing scaffold promoted viability of enclosed hepatocytes and maintained hepatospecific glycogen storage capacity. / Takei, Takayuki; Ijima, Hiroyuki; Sakai, Shinji; Ono, Tsutomu; Kawakami, Koei.

In: Journal of Chemical Engineering of Japan, Vol. 38, No. 11, 20.11.2005, p. 913-917.

Research output: Contribution to journalArticle

@article{e91abe95f86d4c13ad27980eb1dc1202,
title = "Enhanced angiogenesis in bFGF-containing scaffold promoted viability of enclosed hepatocytes and maintained hepatospecific glycogen storage capacity",
abstract = "We fabricated a scaffold of poly (lactic acid) and acidic gelatin, a sustained-release carrier of basic fibroblast growth factor (bFGF), which is a positive regulator of angiogenesis. After 1 week of implantation into mesentery of rats, blood vessels induced in the bFGF-containing scaffolds were almost double those in the bFGF-free scaffolds. Further, histological examination of hepatocyte-immobilizing scaffolds retrieved after 1 week of implantation into the mesentery of the rats that received a 70{\%} hepatectomy revealed that the bFGF-containing scaffolds were more efficient for immobilized cell survival than the bFGF-free scaffolds. As well, hepatocytes in the bFGF-containing scaffolds kept their hepatospecific glycogen storage capacity.",
keywords = "Angiogenesis, bFGF, Cell transplantation, Hepatocyte, Scaffold",
author = "Takayuki Takei and Hiroyuki Ijima and Shinji Sakai and Tsutomu Ono and Koei Kawakami",
year = "2005",
month = "11",
day = "20",
doi = "10.1252/jcej.38.913",
language = "English",
volume = "38",
pages = "913--917",
journal = "Journal of Chemical Engineering of Japan",
issn = "0021-9592",
publisher = "Society of Chemical Engineers, Japan",
number = "11",

}

TY - JOUR

T1 - Enhanced angiogenesis in bFGF-containing scaffold promoted viability of enclosed hepatocytes and maintained hepatospecific glycogen storage capacity

AU - Takei, Takayuki

AU - Ijima, Hiroyuki

AU - Sakai, Shinji

AU - Ono, Tsutomu

AU - Kawakami, Koei

PY - 2005/11/20

Y1 - 2005/11/20

N2 - We fabricated a scaffold of poly (lactic acid) and acidic gelatin, a sustained-release carrier of basic fibroblast growth factor (bFGF), which is a positive regulator of angiogenesis. After 1 week of implantation into mesentery of rats, blood vessels induced in the bFGF-containing scaffolds were almost double those in the bFGF-free scaffolds. Further, histological examination of hepatocyte-immobilizing scaffolds retrieved after 1 week of implantation into the mesentery of the rats that received a 70% hepatectomy revealed that the bFGF-containing scaffolds were more efficient for immobilized cell survival than the bFGF-free scaffolds. As well, hepatocytes in the bFGF-containing scaffolds kept their hepatospecific glycogen storage capacity.

AB - We fabricated a scaffold of poly (lactic acid) and acidic gelatin, a sustained-release carrier of basic fibroblast growth factor (bFGF), which is a positive regulator of angiogenesis. After 1 week of implantation into mesentery of rats, blood vessels induced in the bFGF-containing scaffolds were almost double those in the bFGF-free scaffolds. Further, histological examination of hepatocyte-immobilizing scaffolds retrieved after 1 week of implantation into the mesentery of the rats that received a 70% hepatectomy revealed that the bFGF-containing scaffolds were more efficient for immobilized cell survival than the bFGF-free scaffolds. As well, hepatocytes in the bFGF-containing scaffolds kept their hepatospecific glycogen storage capacity.

KW - Angiogenesis

KW - bFGF

KW - Cell transplantation

KW - Hepatocyte

KW - Scaffold

UR - http://www.scopus.com/inward/record.url?scp=28844455948&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28844455948&partnerID=8YFLogxK

U2 - 10.1252/jcej.38.913

DO - 10.1252/jcej.38.913

M3 - Article

AN - SCOPUS:28844455948

VL - 38

SP - 913

EP - 917

JO - Journal of Chemical Engineering of Japan

JF - Journal of Chemical Engineering of Japan

SN - 0021-9592

IS - 11

ER -