Engineering tumors with 3D scaffolds

Claudia Fischbach, Ruth Chen, Takuya Matsumoto, Tobias Schmelzle, Joan S. Brugge, Peter J. Polverini, David J. Mooney

Research output: Contribution to journalArticle

551 Citations (Scopus)

Abstract

Microenvironmental conditions control tumorigenesis and biomimetic culture systems that allow for in vitro and in vivo tumor modeling may greatly aid studies of cancer cells' dependency on these conditions. We engineered three-dimensional (3D) human tumor models using carcinoma cells in polymeric scaffolds that recreated microenvironmental characteristics representative of tumors in vivo. Strikingly, the angiogenic characteristics of tumor cells were dramatically altered upon 3D culture within this system, and corresponded much more closely to tumors formed in vivo. Cells in this model were also less sensitive to chemotherapy and yielded tumors with enhanced malignant potential. We assessed the broad relevance of these findings with 3D culture of other tumor cell lines in this same model, comparison with standard 3D Matrigel culture and in vivo experiments. This new biomimetic model may provide a broadly applicable 3D culture system to study the effect of microenvironmental conditions on tumor malignancy in vitro and in vivo.

Original languageEnglish
Pages (from-to)855-860
Number of pages6
JournalNature Methods
Volume4
Issue number10
DOIs
Publication statusPublished - Oct 2007
Externally publishedYes

Fingerprint

Scaffolds (biology)
Tumors
Neoplasms
Cells
Biomimetics
Cell culture
Chemotherapy
Scaffolds
Tumor Cell Line
Carcinogenesis
Carcinoma
Drug Therapy

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Cite this

Fischbach, C., Chen, R., Matsumoto, T., Schmelzle, T., Brugge, J. S., Polverini, P. J., & Mooney, D. J. (2007). Engineering tumors with 3D scaffolds. Nature Methods, 4(10), 855-860. https://doi.org/10.1038/nmeth1085

Engineering tumors with 3D scaffolds. / Fischbach, Claudia; Chen, Ruth; Matsumoto, Takuya; Schmelzle, Tobias; Brugge, Joan S.; Polverini, Peter J.; Mooney, David J.

In: Nature Methods, Vol. 4, No. 10, 10.2007, p. 855-860.

Research output: Contribution to journalArticle

Fischbach, C, Chen, R, Matsumoto, T, Schmelzle, T, Brugge, JS, Polverini, PJ & Mooney, DJ 2007, 'Engineering tumors with 3D scaffolds', Nature Methods, vol. 4, no. 10, pp. 855-860. https://doi.org/10.1038/nmeth1085
Fischbach C, Chen R, Matsumoto T, Schmelzle T, Brugge JS, Polverini PJ et al. Engineering tumors with 3D scaffolds. Nature Methods. 2007 Oct;4(10):855-860. https://doi.org/10.1038/nmeth1085
Fischbach, Claudia ; Chen, Ruth ; Matsumoto, Takuya ; Schmelzle, Tobias ; Brugge, Joan S. ; Polverini, Peter J. ; Mooney, David J. / Engineering tumors with 3D scaffolds. In: Nature Methods. 2007 ; Vol. 4, No. 10. pp. 855-860.
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