Engineering rice with lower grain arsenic

Fenglin Deng, Naoki Yamaji, Jian Feng Ma, Sang Kyu Lee, Jong Seong Jeon, Enrico Martinoia, Youngsook Lee, Won Yong Song

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Arsenic (As) is a poisonous element that causes severe skin lesions and cancer in humans. Rice (Oryza sativa L.) is a major dietary source of As in humans who consume this cereal as a staple food. We hypothesized that increasing As vacuolar sequestration would inhibit its translocation into the grain and reduce the amount of As entering the food chain. We developed transgenic rice plants expressing two different vacuolar As sequestration genes, ScYCF1 and OsABCC1, under the control of the RCc3 promoter in the root cortical and internode phloem cells, along with a bacterial γ-glutamylcysteine synthetase driven by the maize UBI promoter. The transgenic rice plants exhibited reduced root-to-shoot and internode-to-grain As translocation, resulting in a 70% reduction in As accumulation in the brown rice without jeopardizing agronomic traits. This technology could be used to reduce As intake, particularly in populations of South East Asia suffering from As toxicity and thereby improve human health.

Original languageEnglish
JournalPlant Biotechnology Journal
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Arsenic
arsenic
engineering
rice
Genetically Modified Plants
internodes
promoter regions
genetically modified organisms
Glutamate-Cysteine Ligase
Phloem
Oryza
Food Chain
brown rice
Far East
staple foods
Skin Neoplasms
skin lesions
ligases
South East Asia
food chain

Keywords

  • ABC transporter
  • Arsenic
  • Rice
  • Vacuolar sequestration

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Deng, F., Yamaji, N., Ma, J. F., Lee, S. K., Jeon, J. S., Martinoia, E., ... Song, W. Y. (Accepted/In press). Engineering rice with lower grain arsenic. Plant Biotechnology Journal. https://doi.org/10.1111/pbi.12905

Engineering rice with lower grain arsenic. / Deng, Fenglin; Yamaji, Naoki; Ma, Jian Feng; Lee, Sang Kyu; Jeon, Jong Seong; Martinoia, Enrico; Lee, Youngsook; Song, Won Yong.

In: Plant Biotechnology Journal, 01.01.2018.

Research output: Contribution to journalArticle

Deng, Fenglin ; Yamaji, Naoki ; Ma, Jian Feng ; Lee, Sang Kyu ; Jeon, Jong Seong ; Martinoia, Enrico ; Lee, Youngsook ; Song, Won Yong. / Engineering rice with lower grain arsenic. In: Plant Biotechnology Journal. 2018.
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