TY - JOUR
T1 - Energy transfer processes in chlorophyll f-containing cyanobacteria using time-resolved fluorescence spectroscopy on intact cells
AU - Tomo, Tatsuya
AU - Shinoda, Toshiyuki
AU - Chen, Min
AU - Allakhverdiev, Suleyman I.
AU - Akimoto, Seiji
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education of Japan ( 21570038 to T.T., 22370017 to T.T. and S.A., and 23370013 to S.A.), a grant from JST PRESTO (T.T.), and a grant from the Australian Research Council's Discovery Projects funding scheme (project number DP12101360 to T.T.). S.I.A. was supported by grants from the Russian Science Foundation . M.C. holds an Australian Research Council Future Fellowship, and M.C. thanks the Australian Research Council for support.
PY - 2014/9
Y1 - 2014/9
N2 - We examined energy transfer dynamics in the unique chlorophyll (Chl) f-containing cyanobacterium Halomicronema hongdechloris. The absorption band of Chl f appeared during cultivation of this organism under far-red light. The absorption maximum of Chl f in organic solvents occurs at a wavelength of approximately 40 nm longer than that of Chl a. In vivo, the cells display a new absorption band at approximately 730 nm at 298 K, which is at a significantly longer wavelength than that of Chl a. We primarily assigned this band to a long wavelength form of Chl a. The function of Chl f is currently unknown. We measured the fluorescence of cells using time-resolved fluorescence spectroscopy in the picosecond-to-nanosecond time range and found clear differences in fluorescence properties between the cells that contained Chl f and the cells that did not. After excitation, the fluorescence peaks of photosystem I and photosystem II appeared quickly but diminished immediately. A unique fluorescence peak located at 748 nm subsequently appeared in cells containing Chl f. This finding strongly suggests that the Chl f in this alga exists in photosystem I and II complexes and is located close to each molecule of Chl a. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy.
AB - We examined energy transfer dynamics in the unique chlorophyll (Chl) f-containing cyanobacterium Halomicronema hongdechloris. The absorption band of Chl f appeared during cultivation of this organism under far-red light. The absorption maximum of Chl f in organic solvents occurs at a wavelength of approximately 40 nm longer than that of Chl a. In vivo, the cells display a new absorption band at approximately 730 nm at 298 K, which is at a significantly longer wavelength than that of Chl a. We primarily assigned this band to a long wavelength form of Chl a. The function of Chl f is currently unknown. We measured the fluorescence of cells using time-resolved fluorescence spectroscopy in the picosecond-to-nanosecond time range and found clear differences in fluorescence properties between the cells that contained Chl f and the cells that did not. After excitation, the fluorescence peaks of photosystem I and photosystem II appeared quickly but diminished immediately. A unique fluorescence peak located at 748 nm subsequently appeared in cells containing Chl f. This finding strongly suggests that the Chl f in this alga exists in photosystem I and II complexes and is located close to each molecule of Chl a. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy.
KW - Chlorophyll f
KW - Energy transfer
KW - Fluorescence
KW - Photosynthesis
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U2 - 10.1016/j.bbabio.2014.04.009
DO - 10.1016/j.bbabio.2014.04.009
M3 - Article
C2 - 24792349
AN - SCOPUS:84906303201
VL - 1837
SP - 1484
EP - 1489
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
SN - 0005-2728
IS - 9
ER -