Energy transfer processes in chlorophyll f-containing cyanobacteria using time-resolved fluorescence spectroscopy on intact cells

Tatsuya Tomo, Toshiyuki Shinoda, Min Chen, Suleyman I. Allakhverdiev, Seiji Akimoto

    Research output: Contribution to journalArticlepeer-review

    23 Citations (Scopus)

    Abstract

    We examined energy transfer dynamics in the unique chlorophyll (Chl) f-containing cyanobacterium Halomicronema hongdechloris. The absorption band of Chl f appeared during cultivation of this organism under far-red light. The absorption maximum of Chl f in organic solvents occurs at a wavelength of approximately 40 nm longer than that of Chl a. In vivo, the cells display a new absorption band at approximately 730 nm at 298 K, which is at a significantly longer wavelength than that of Chl a. We primarily assigned this band to a long wavelength form of Chl a. The function of Chl f is currently unknown. We measured the fluorescence of cells using time-resolved fluorescence spectroscopy in the picosecond-to-nanosecond time range and found clear differences in fluorescence properties between the cells that contained Chl f and the cells that did not. After excitation, the fluorescence peaks of photosystem I and photosystem II appeared quickly but diminished immediately. A unique fluorescence peak located at 748 nm subsequently appeared in cells containing Chl f. This finding strongly suggests that the Chl f in this alga exists in photosystem I and II complexes and is located close to each molecule of Chl a. This article is part of a Special Issue entitled: Photosynthesis Research for Sustainability: Keys to Produce Clean Energy.

    Original languageEnglish
    Pages (from-to)1484-1489
    Number of pages6
    JournalBiochimica et Biophysica Acta - Bioenergetics
    Volume1837
    Issue number9
    DOIs
    Publication statusPublished - Sep 2014

    Keywords

    • Chlorophyll f
    • Energy transfer
    • Fluorescence
    • Photosynthesis

    ASJC Scopus subject areas

    • Biophysics
    • Biochemistry
    • Cell Biology

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