Energy transfer in the chlorophyll f-containing cyanobacterium, Halomicronema hongdechloris, analyzed by time-resolved fluorescence spectroscopies

Seiji Akimoto, Toshiyuki Shinoda, Min Chen, Suleyman I. Allakhverdiev, Tatsuya Tomo

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We prepared thylakoid membranes from Halomicronema hongdechloris cells grown under white fluorescent light or light from far-red (740 nm) light-emitting diodes, and observed their energy-transfer processes shortly after light excitation. Excitation-relaxation processes were examined by steady-state and time-resolved fluorescence spectroscopies. Two time-resolved fluorescence techniques were used: time-correlated single photon counting and fluorescence up-conversion methods. The thylakoids from the cells grown under white light contained chlorophyll (Chl) a of different energies, but were devoid of Chl f. At room temperature, the excitation energy was equilibrated among the Chl a pools with a time constant of 6.6 ps. Conversely, the thylakoids from the cells grown under far-red light possessed both Chl a and Chl f. Two energy-transfer pathways from Chl a to Chl f were identified with time constants of 1.3 and 5.0 ps, and the excitation energy was equilibrated between the Chl a and Chl f pools at room temperature. We also examined the energy-transfer pathways from phycobilisome to the two photosystems under white-light cultivation.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalPhotosynthesis research
Volume125
Issue number1-2
DOIs
Publication statusPublished - Aug 17 2015

Keywords

  • Chlorophyll f
  • Energy transfer
  • Fluorescence
  • Light adaptation
  • Pigment-protein complex
  • Time-resolved spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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