Energy transfer in the chlorophyll f-containing cyanobacterium, Halomicronema hongdechloris, analyzed by time-resolved fluorescence spectroscopies

Seiji Akimoto, Toshiyuki Shinoda, Min Chen, Suleyman Allakhverdiev, Tatsuya Tomo

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We prepared thylakoid membranes from Halomicronema hongdechloris cells grown under white fluorescent light or light from far-red (740 nm) light-emitting diodes, and observed their energy-transfer processes shortly after light excitation. Excitation-relaxation processes were examined by steady-state and time-resolved fluorescence spectroscopies. Two time-resolved fluorescence techniques were used: time-correlated single photon counting and fluorescence up-conversion methods. The thylakoids from the cells grown under white light contained chlorophyll (Chl) a of different energies, but were devoid of Chl f. At room temperature, the excitation energy was equilibrated among the Chl a pools with a time constant of 6.6 ps. Conversely, the thylakoids from the cells grown under far-red light possessed both Chl a and Chl f. Two energy-transfer pathways from Chl a to Chl f were identified with time constants of 1.3 and 5.0 ps, and the excitation energy was equilibrated between the Chl a and Chl f pools at room temperature. We also examined the energy-transfer pathways from phycobilisome to the two photosystems under white-light cultivation.

Original languageEnglish
Pages (from-to)115-122
Number of pages8
JournalPhotosynthesis Research
Volume125
Issue number1-2
DOIs
Publication statusPublished - Aug 17 2015
Externally publishedYes

Fingerprint

fluorescence emission spectroscopy
Fluorescence Spectrometry
Energy Transfer
Fluorescence spectroscopy
Cyanobacteria
energy transfer
Energy transfer
chlorophyll
Light
Thylakoids
Excitation energy
Cells
thylakoids
Fluorescence
white light
Phycobilisomes
ambient temperature
energy
Relaxation processes
Temperature

Keywords

  • Chlorophyll f
  • Energy transfer
  • Fluorescence
  • Light adaptation
  • Pigment-protein complex
  • Time-resolved spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Energy transfer in the chlorophyll f-containing cyanobacterium, Halomicronema hongdechloris, analyzed by time-resolved fluorescence spectroscopies. / Akimoto, Seiji; Shinoda, Toshiyuki; Chen, Min; Allakhverdiev, Suleyman; Tomo, Tatsuya.

In: Photosynthesis Research, Vol. 125, No. 1-2, 17.08.2015, p. 115-122.

Research output: Contribution to journalArticle

Akimoto, Seiji ; Shinoda, Toshiyuki ; Chen, Min ; Allakhverdiev, Suleyman ; Tomo, Tatsuya. / Energy transfer in the chlorophyll f-containing cyanobacterium, Halomicronema hongdechloris, analyzed by time-resolved fluorescence spectroscopies. In: Photosynthesis Research. 2015 ; Vol. 125, No. 1-2. pp. 115-122.
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