Energy coupling of L-glutamate transport and vacuolar H+-ATPf in brain synaptic vesicles

Yoshinori Moriyama, Masatomo Maeda, Masamitsu Futai, Akira Hachimori

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Energy coupling of L-glutamate transport in brain synaptic vesicles has been studied. ATP-dependent acidification of the bovine brain synaptic vesicles was shown to require Cl-, to be accelerated by valinomycin and to be abolished by ammonium sulfate, nigericin or CCCP plus valinomycin, and K+. On the other hand, ATP-driven formation of a membrane potential (positive inside) was found to be stimulated by ammonium sulfate, not to be affected by nigericin and to be abolished by CCCP plus valinomycin and K+. Like formation of a membrane potential, ATP-dependent L-[3H]glutamate uptake into vesicles was stimulated by ammonium sulfate, not affected by nigericin and abolished by CCCP plus valinomycin and K+. The L- [3H]glutamate uptake differed in specificity from the transport system in synaptic plasma membranes. Both ATP-dependent H+ pump activity and L-glutamate uptake were inhibited by bafilomycin and cold treatment (common properties of vacuolar H-ATPase). ATP-dependent acidification in the presence of L-glutamate was also observed, suggesting that L-glutamate uptake lowered the membrane potential to drive further entry of H+. These results were consistent with the notion that the vacuolar H+-ATPase of synaptic vesicles formed a membrane potential to drive L-glutamate uptake. ATPase activity of the vesicles was not affected by the addition of Cl-, glutamate or nigericin, indicating that an electrochemical H+ gradient had no effect on the ATPase activity.

Original languageEnglish
Pages (from-to)689-693
Number of pages5
JournalJournal of biochemistry
Volume108
Issue number4
DOIs
Publication statusPublished - Oct 1990

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Energy coupling of L-glutamate transport and vacuolar H<sup>+</sup>-ATPf in brain synaptic vesicles'. Together they form a unique fingerprint.

  • Cite this