Endothelin receptors in ischemic rat brain and alzheimer brain

M. Kohzuki, H. Onodera, M. Yasujima, Y. Itoyama, M. Kanazawa, T. Sato, Koji Abe

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Endothelin (ET) binding sites in male Wistar rat brains subjected to a 20-min four-vessel occlusion (transient forebrain ischemia model) which induces hip-pocampal neuron death, and in human brains with Alzheimer disease, were mapped by quantitative in vitro autoradiography employing [125I]ET-1 as a radioligand. Rats were decapitated 4 or 7 days after ischemia. In the rat brain, the [125I]ET-1 binding sites were remarkably increased in the hippocampal CA1 and dentate gyrus, ventral thalamic nucleus, and cortical vessels 4 and 7 days after ischemia, when many reactive astroglia were observed. The [125I]ET-1 binding sites decreased in the cerebral cortex affected by Alzheimer disease. The binding was abolished by 1 μM unlabeled ET-1, ET-3, sarafotoxin S6b, and BQ788 (an ETBantagonist) but not by BQ123 (an ETAantagonist), suggesting that the [125I]ET-1 binding sites are as ETBreceptors. The present findings raise the possibility that a glial ET system could be responsible for the occurrence of ischemic neuron cell death.

Original languageEnglish
Pages (from-to)S329-S331
JournalJournal of Cardiovascular Pharmacology
Volume26
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Endothelin Receptors
Endothelin-1
Binding Sites
Brain
Ischemia
Endothelins
Alzheimer Disease
Endothelin-3
Ventral Thalamic Nuclei
Neurons
Parahippocampal Gyrus
Dentate Gyrus
Prosencephalon
Autoradiography
Neuroglia
Astrocytes
Cerebral Cortex
Wistar Rats
Hip
Cell Death

Keywords

  • Alzheimer
  • Endothelin receptor
  • Human
  • Ischemia

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Pharmacology

Cite this

Kohzuki, M., Onodera, H., Yasujima, M., Itoyama, Y., Kanazawa, M., Sato, T., & Abe, K. (1995). Endothelin receptors in ischemic rat brain and alzheimer brain. Journal of Cardiovascular Pharmacology, 26, S329-S331.

Endothelin receptors in ischemic rat brain and alzheimer brain. / Kohzuki, M.; Onodera, H.; Yasujima, M.; Itoyama, Y.; Kanazawa, M.; Sato, T.; Abe, Koji.

In: Journal of Cardiovascular Pharmacology, Vol. 26, 1995, p. S329-S331.

Research output: Contribution to journalArticle

Kohzuki, M, Onodera, H, Yasujima, M, Itoyama, Y, Kanazawa, M, Sato, T & Abe, K 1995, 'Endothelin receptors in ischemic rat brain and alzheimer brain', Journal of Cardiovascular Pharmacology, vol. 26, pp. S329-S331.
Kohzuki M, Onodera H, Yasujima M, Itoyama Y, Kanazawa M, Sato T et al. Endothelin receptors in ischemic rat brain and alzheimer brain. Journal of Cardiovascular Pharmacology. 1995;26:S329-S331.
Kohzuki, M. ; Onodera, H. ; Yasujima, M. ; Itoyama, Y. ; Kanazawa, M. ; Sato, T. ; Abe, Koji. / Endothelin receptors in ischemic rat brain and alzheimer brain. In: Journal of Cardiovascular Pharmacology. 1995 ; Vol. 26. pp. S329-S331.
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