Endothelial nitric oxide synthase-11R protein therapy for prevention of cerebral vasospasm in rats: A preliminary report

Tomoyuki Ogawa, S. Ono, T. Ichikawa, S. Arimitsu, K. Onoda, K. Tokunaga, Kenji Sugiu, Kazuhito Tomizawa, Hideki Matsui, Isao Date

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In one of our studies we found that enhanced green fluorescent protein (EGFP) fused with consecutive 11 arginines (11R), one of the protein transduction domains (PTDs) [1-6, 11], and effectively penetrated into all layers of the rat basilar artery (BA). We examined whether eNOS (140-kDa) fused 11R (11R-eNOS) was also transduced into the BAs and had a positive effect on the attenuation of cerebral vasospasm. 11R-eNOS or saline was injected into the cisterna magna of male Sprague-Dawley rats. Two hours after the injection, the BAs were extracted from the rats and transduction efficacy of 11R-eNOS in the BA was evaluated by immunofluorescence staining. To examine the effect of 11R-eNOS on the cerebral arteries exposed to SAH, we measured the post SAH BA diameters six hours after the injection of 11R-eNOS. Immunofluorescent study confirmed the presence of 11R-eNOS protein in the layers of the cerebral arteries in vivo. 11R-eNOS had a positive effect on attenuation of cerebral vasospasm. 11R-eNOS was effectively transduced into the walls of the BA. 11R-eNOS inhibited the vasoconstriction after SAH. These results suggest that 11R-eNOS protein therapy has a potential in treating cerebral vasospasm.

Original languageEnglish
Title of host publicationActa Neurochirurgica, Supplementum
Pages165-167
Number of pages3
Edition104
DOIs
Publication statusPublished - 2008

Publication series

NameActa Neurochirurgica, Supplementum
Number104
ISSN (Print)00651419

Fingerprint

Intracranial Vasospasm
Basilar Artery
Nitric Oxide Synthase Type III
Cerebral Arteries
Proteins
Cisterna Magna
Injections
Therapeutics
Vasoconstriction
Fluorescent Antibody Technique
Sprague Dawley Rats
Arginine
Staining and Labeling

Keywords

  • 11 arginines
  • Cerebral vasospasm
  • nitric oxide synthase
  • protein transduction domain
  • subarachnoid haemorrhage

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Ogawa, T., Ono, S., Ichikawa, T., Arimitsu, S., Onoda, K., Tokunaga, K., ... Date, I. (2008). Endothelial nitric oxide synthase-11R protein therapy for prevention of cerebral vasospasm in rats: A preliminary report. In Acta Neurochirurgica, Supplementum (104 ed., pp. 165-167). (Acta Neurochirurgica, Supplementum; No. 104). https://doi.org/10.1007/978-3-211-75718-5-32

Endothelial nitric oxide synthase-11R protein therapy for prevention of cerebral vasospasm in rats : A preliminary report. / Ogawa, Tomoyuki; Ono, S.; Ichikawa, T.; Arimitsu, S.; Onoda, K.; Tokunaga, K.; Sugiu, Kenji; Tomizawa, Kazuhito; Matsui, Hideki; Date, Isao.

Acta Neurochirurgica, Supplementum. 104. ed. 2008. p. 165-167 (Acta Neurochirurgica, Supplementum; No. 104).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ogawa, T, Ono, S, Ichikawa, T, Arimitsu, S, Onoda, K, Tokunaga, K, Sugiu, K, Tomizawa, K, Matsui, H & Date, I 2008, Endothelial nitric oxide synthase-11R protein therapy for prevention of cerebral vasospasm in rats: A preliminary report. in Acta Neurochirurgica, Supplementum. 104 edn, Acta Neurochirurgica, Supplementum, no. 104, pp. 165-167. https://doi.org/10.1007/978-3-211-75718-5-32
Ogawa T, Ono S, Ichikawa T, Arimitsu S, Onoda K, Tokunaga K et al. Endothelial nitric oxide synthase-11R protein therapy for prevention of cerebral vasospasm in rats: A preliminary report. In Acta Neurochirurgica, Supplementum. 104 ed. 2008. p. 165-167. (Acta Neurochirurgica, Supplementum; 104). https://doi.org/10.1007/978-3-211-75718-5-32
Ogawa, Tomoyuki ; Ono, S. ; Ichikawa, T. ; Arimitsu, S. ; Onoda, K. ; Tokunaga, K. ; Sugiu, Kenji ; Tomizawa, Kazuhito ; Matsui, Hideki ; Date, Isao. / Endothelial nitric oxide synthase-11R protein therapy for prevention of cerebral vasospasm in rats : A preliminary report. Acta Neurochirurgica, Supplementum. 104. ed. 2008. pp. 165-167 (Acta Neurochirurgica, Supplementum; 104).
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