Endogytosis of cationic iron colloid particles by rat peritoneal macrophages and the ferritin synthesis. A morphological study

Toshiro Ono, Satimaru Seno, Tadashi Tsujii

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Observations were made on rat peritoneal macrophages exposed to cationic ferric hydroxide colloid (ferric hydroxide-cacodylate complex, Fe-Cac) in vitro in order to study the process of endocytosis of the cationic particles and their fate after ingestion. Fe-Cac particles given to macrophages were adhered to the cell surface and taken up through endocytosis. The ingested particles were first found in the cytoplasmic tiny vesicles and canals adhering to their inner surfaces. These tiny vesicles seldom fused each other or with lysosomes at the early stage of incubation. By further incubation in the iron-free medium, however, macrophages developed the acid phosphatase positive large vacuoles containing a quantity of iron particles, showing the fusion of some Fe-Cac containing endocytic vacuoles with lysosomes. After 15 hr of chase in iron-free medium ferritin molecules in paracrystalline arrangements appeared in a few siderosomes and also diffusely scattered ones in cytoplasmic matrix. Immunocytochemical observations of ferritin with antirat ferritin antibody and FITC conjugated rabbit IgG showed diffuse staining of cytoplasm after 10 to 15 hr of chase in iron-free medium.

Original languageEnglish
Pages (from-to)515-524
Number of pages10
JournalActa Histochemica et Cytochemica
Volume18
Issue number5
DOIs
Publication statusPublished - 1985
Externally publishedYes

Fingerprint

Macrophages
Peritoneal Macrophages
Colloids
Cacodylic Acid
Ferritins
Rats
Iron
Endocytosis
Vacuoles
Lysosomes
Cytoplasmic Vesicles
Fluorescein-5-isothiocyanate
Canals
Acid Phosphatase
Cytoplasm
Fusion reactions
Eating
Immunoglobulin G
Staining and Labeling
Rabbits

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Physiology
  • Biochemistry
  • Cell Biology

Cite this

Endogytosis of cationic iron colloid particles by rat peritoneal macrophages and the ferritin synthesis. A morphological study. / Ono, Toshiro; Seno, Satimaru; Tsujii, Tadashi.

In: Acta Histochemica et Cytochemica, Vol. 18, No. 5, 1985, p. 515-524.

Research output: Contribution to journalArticle

@article{ec5fd6daf92a44acbdfb001337e5af31,
title = "Endogytosis of cationic iron colloid particles by rat peritoneal macrophages and the ferritin synthesis. A morphological study",
abstract = "Observations were made on rat peritoneal macrophages exposed to cationic ferric hydroxide colloid (ferric hydroxide-cacodylate complex, Fe-Cac) in vitro in order to study the process of endocytosis of the cationic particles and their fate after ingestion. Fe-Cac particles given to macrophages were adhered to the cell surface and taken up through endocytosis. The ingested particles were first found in the cytoplasmic tiny vesicles and canals adhering to their inner surfaces. These tiny vesicles seldom fused each other or with lysosomes at the early stage of incubation. By further incubation in the iron-free medium, however, macrophages developed the acid phosphatase positive large vacuoles containing a quantity of iron particles, showing the fusion of some Fe-Cac containing endocytic vacuoles with lysosomes. After 15 hr of chase in iron-free medium ferritin molecules in paracrystalline arrangements appeared in a few siderosomes and also diffusely scattered ones in cytoplasmic matrix. Immunocytochemical observations of ferritin with antirat ferritin antibody and FITC conjugated rabbit IgG showed diffuse staining of cytoplasm after 10 to 15 hr of chase in iron-free medium.",
author = "Toshiro Ono and Satimaru Seno and Tadashi Tsujii",
year = "1985",
doi = "10.1267/ahc.18.515",
language = "English",
volume = "18",
pages = "515--524",
journal = "Acta Histochemica et Cytochemica",
issn = "0044-5991",
publisher = "Japan Society of Histochemistry and Cytochemistry",
number = "5",

}

TY - JOUR

T1 - Endogytosis of cationic iron colloid particles by rat peritoneal macrophages and the ferritin synthesis. A morphological study

AU - Ono, Toshiro

AU - Seno, Satimaru

AU - Tsujii, Tadashi

PY - 1985

Y1 - 1985

N2 - Observations were made on rat peritoneal macrophages exposed to cationic ferric hydroxide colloid (ferric hydroxide-cacodylate complex, Fe-Cac) in vitro in order to study the process of endocytosis of the cationic particles and their fate after ingestion. Fe-Cac particles given to macrophages were adhered to the cell surface and taken up through endocytosis. The ingested particles were first found in the cytoplasmic tiny vesicles and canals adhering to their inner surfaces. These tiny vesicles seldom fused each other or with lysosomes at the early stage of incubation. By further incubation in the iron-free medium, however, macrophages developed the acid phosphatase positive large vacuoles containing a quantity of iron particles, showing the fusion of some Fe-Cac containing endocytic vacuoles with lysosomes. After 15 hr of chase in iron-free medium ferritin molecules in paracrystalline arrangements appeared in a few siderosomes and also diffusely scattered ones in cytoplasmic matrix. Immunocytochemical observations of ferritin with antirat ferritin antibody and FITC conjugated rabbit IgG showed diffuse staining of cytoplasm after 10 to 15 hr of chase in iron-free medium.

AB - Observations were made on rat peritoneal macrophages exposed to cationic ferric hydroxide colloid (ferric hydroxide-cacodylate complex, Fe-Cac) in vitro in order to study the process of endocytosis of the cationic particles and their fate after ingestion. Fe-Cac particles given to macrophages were adhered to the cell surface and taken up through endocytosis. The ingested particles were first found in the cytoplasmic tiny vesicles and canals adhering to their inner surfaces. These tiny vesicles seldom fused each other or with lysosomes at the early stage of incubation. By further incubation in the iron-free medium, however, macrophages developed the acid phosphatase positive large vacuoles containing a quantity of iron particles, showing the fusion of some Fe-Cac containing endocytic vacuoles with lysosomes. After 15 hr of chase in iron-free medium ferritin molecules in paracrystalline arrangements appeared in a few siderosomes and also diffusely scattered ones in cytoplasmic matrix. Immunocytochemical observations of ferritin with antirat ferritin antibody and FITC conjugated rabbit IgG showed diffuse staining of cytoplasm after 10 to 15 hr of chase in iron-free medium.

UR - http://www.scopus.com/inward/record.url?scp=85007726535&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85007726535&partnerID=8YFLogxK

U2 - 10.1267/ahc.18.515

DO - 10.1267/ahc.18.515

M3 - Article

AN - SCOPUS:85007726535

VL - 18

SP - 515

EP - 524

JO - Acta Histochemica et Cytochemica

JF - Acta Histochemica et Cytochemica

SN - 0044-5991

IS - 5

ER -