Endogenous MCP-1 influences systemic cytokine balance in a murine model of acute septic peritonitis

Akihiro Matsukawa, Cory M. Hogaboam, Nickolas W. Lukacs, Pamela M. Lincoln, Robert M. Strieter, Steven L. Kunkel

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Sepsis and septic syndrome represent an intense systemic response with multiple physiologic and immunologic abnormalities, leading to multiple organ failure. Recent investigations suggest that the critical conditions are balanced by endogenous cytokines. In the present study, we examined the involvement of endogenous monocyte chemoattractant protein (MCP)-1 in the regulation of cytokine production in tissue/organs in a murine model of acute septic peritonitis induced by cecal ligation and puncture (CLP). Initial studies showed that CLP induced elevated levels of MCP-1 in tissues, such as liver, lung, and kidney. To neutralize endogenous MCP-1, either anti-MCP-1 antibodies or control antibodies were intraperitoneally administered 2 h prior to CLP. Administration of anti-MCP-1 antibodies resulted in a decrease in the level of interleukin (IL)-13 in tissues, while increasing the level of tumor necrosis factor-α, compared to control. In addition, anti-MCP-1 treatment decreased the level of IL-12 and, in contrast, increased the level of IL-10 in specific tissues. These findings suggest that endogenous MCP-1 influences the cytokine balance in tissues in favor of antiinflammatory and immune-enhancing cytokines, probably protecting the host from tissue/organ damage during sepsis. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)77-84
Number of pages8
JournalExperimental and Molecular Pathology
Volume68
Issue number2
DOIs
Publication statusPublished - Apr 2000
Externally publishedYes

Fingerprint

Chemokine CCL2
Peritonitis
Cytokines
Tissue
Punctures
Ligation
Antibodies
Systemic Inflammatory Response Syndrome
Interleukin-13
Multiple Organ Failure
Interleukin-12
Liver
Interleukin-10
Sepsis
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Kidney
Lung

Keywords

  • Cytokine balance
  • Monocyte chemoattractant protein-1
  • Multiple organ failure
  • Sepsis

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

Endogenous MCP-1 influences systemic cytokine balance in a murine model of acute septic peritonitis. / Matsukawa, Akihiro; Hogaboam, Cory M.; Lukacs, Nickolas W.; Lincoln, Pamela M.; Strieter, Robert M.; Kunkel, Steven L.

In: Experimental and Molecular Pathology, Vol. 68, No. 2, 04.2000, p. 77-84.

Research output: Contribution to journalArticle

Matsukawa, Akihiro ; Hogaboam, Cory M. ; Lukacs, Nickolas W. ; Lincoln, Pamela M. ; Strieter, Robert M. ; Kunkel, Steven L. / Endogenous MCP-1 influences systemic cytokine balance in a murine model of acute septic peritonitis. In: Experimental and Molecular Pathology. 2000 ; Vol. 68, No. 2. pp. 77-84.
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