Endogenous Inhibitors of Factor B

Takeshi Moriyama, Yasuko Kawamoto, Hidetaka Ichikawa, Keinosuke Okamoto, Takashi Inoue, Tatsuhiko Kobayashi, Akio Miyama

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Abstract

Proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse‐established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by preparative SDS‐polyacrylamide gel electrophoresis, with molecular weights of 25K, 28K, 33K, and 35K. The individual proteins formed a complex with purified mouse factor B at a molecular ratio of 1: 1 and inhibited its hemolytic activity. Proteins 25K and 28K inhibited the hemolytic activity of an activated form of factor B combined with cobra venom factor as well as that of the native form. These inhibitors did not affect the hemolytic activity of the second component of complement in mouse serum. The inhibitory activity of the 25K protein was partially inhibited by antiserum raised against it in rabbits.

Original languageEnglish
Pages (from-to)1079-1091
Number of pages13
JournalMICROBIOLOGY and IMMUNOLOGY
Volume27
Issue number12
DOIs
Publication statusPublished - Dec 1983

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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    Moriyama, T., Kawamoto, Y., Ichikawa, H., Okamoto, K., Inoue, T., Kobayashi, T., & Miyama, A. (1983). Endogenous Inhibitors of Factor B. MICROBIOLOGY and IMMUNOLOGY, 27(12), 1079-1091. https://doi.org/10.1111/j.1348-0421.1983.tb02941.x