Endogenous Inhibitors of Factor B

Takeshi Moriyama, Yasuko Kawamoto, Hidetaka Ichikawa, Keinosuke Okamoto, Takashi Inoue, Tatsuhiko Kobayashi, Akio Miyama

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse‐established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by preparative SDS‐polyacrylamide gel electrophoresis, with molecular weights of 25K, 28K, 33K, and 35K. The individual proteins formed a complex with purified mouse factor B at a molecular ratio of 1: 1 and inhibited its hemolytic activity. Proteins 25K and 28K inhibited the hemolytic activity of an activated form of factor B combined with cobra venom factor as well as that of the native form. These inhibitors did not affect the hemolytic activity of the second component of complement in mouse serum. The inhibitory activity of the 25K protein was partially inhibited by antiserum raised against it in rabbits.

Original languageEnglish
Pages (from-to)1079-1091
Number of pages13
JournalMicrobiology and Immunology
Volume27
Issue number12
DOIs
Publication statusPublished - 1983
Externally publishedYes

Fingerprint

Proteins
Peritoneal Macrophages
Electrophoresis
Immune Sera
Molecular Weight
Gels
Rabbits
Serum
cobra venom factor

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

Cite this

Moriyama, T., Kawamoto, Y., Ichikawa, H., Okamoto, K., Inoue, T., Kobayashi, T., & Miyama, A. (1983). Endogenous Inhibitors of Factor B. Microbiology and Immunology, 27(12), 1079-1091. https://doi.org/10.1111/j.1348-0421.1983.tb02941.x

Endogenous Inhibitors of Factor B. / Moriyama, Takeshi; Kawamoto, Yasuko; Ichikawa, Hidetaka; Okamoto, Keinosuke; Inoue, Takashi; Kobayashi, Tatsuhiko; Miyama, Akio.

In: Microbiology and Immunology, Vol. 27, No. 12, 1983, p. 1079-1091.

Research output: Contribution to journalArticle

Moriyama, T, Kawamoto, Y, Ichikawa, H, Okamoto, K, Inoue, T, Kobayashi, T & Miyama, A 1983, 'Endogenous Inhibitors of Factor B', Microbiology and Immunology, vol. 27, no. 12, pp. 1079-1091. https://doi.org/10.1111/j.1348-0421.1983.tb02941.x
Moriyama T, Kawamoto Y, Ichikawa H, Okamoto K, Inoue T, Kobayashi T et al. Endogenous Inhibitors of Factor B. Microbiology and Immunology. 1983;27(12):1079-1091. https://doi.org/10.1111/j.1348-0421.1983.tb02941.x
Moriyama, Takeshi ; Kawamoto, Yasuko ; Ichikawa, Hidetaka ; Okamoto, Keinosuke ; Inoue, Takashi ; Kobayashi, Tatsuhiko ; Miyama, Akio. / Endogenous Inhibitors of Factor B. In: Microbiology and Immunology. 1983 ; Vol. 27, No. 12. pp. 1079-1091.
@article{7043e9adaf494715a567c09a8d00cebf,
title = "Endogenous Inhibitors of Factor B",
abstract = "Proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse‐established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by preparative SDS‐polyacrylamide gel electrophoresis, with molecular weights of 25K, 28K, 33K, and 35K. The individual proteins formed a complex with purified mouse factor B at a molecular ratio of 1: 1 and inhibited its hemolytic activity. Proteins 25K and 28K inhibited the hemolytic activity of an activated form of factor B combined with cobra venom factor as well as that of the native form. These inhibitors did not affect the hemolytic activity of the second component of complement in mouse serum. The inhibitory activity of the 25K protein was partially inhibited by antiserum raised against it in rabbits.",
author = "Takeshi Moriyama and Yasuko Kawamoto and Hidetaka Ichikawa and Keinosuke Okamoto and Takashi Inoue and Tatsuhiko Kobayashi and Akio Miyama",
year = "1983",
doi = "10.1111/j.1348-0421.1983.tb02941.x",
language = "English",
volume = "27",
pages = "1079--1091",
journal = "Microbiology and Immunology",
issn = "0385-5600",
publisher = "Center for Academic Publications Japan",
number = "12",

}

TY - JOUR

T1 - Endogenous Inhibitors of Factor B

AU - Moriyama, Takeshi

AU - Kawamoto, Yasuko

AU - Ichikawa, Hidetaka

AU - Okamoto, Keinosuke

AU - Inoue, Takashi

AU - Kobayashi, Tatsuhiko

AU - Miyama, Akio

PY - 1983

Y1 - 1983

N2 - Proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse‐established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by preparative SDS‐polyacrylamide gel electrophoresis, with molecular weights of 25K, 28K, 33K, and 35K. The individual proteins formed a complex with purified mouse factor B at a molecular ratio of 1: 1 and inhibited its hemolytic activity. Proteins 25K and 28K inhibited the hemolytic activity of an activated form of factor B combined with cobra venom factor as well as that of the native form. These inhibitors did not affect the hemolytic activity of the second component of complement in mouse serum. The inhibitory activity of the 25K protein was partially inhibited by antiserum raised against it in rabbits.

AB - Proteins which were able to bind noncovalently with mouse factor B were found in cells that are nonsecretors of factor B such as mouse‐established monocytic cells and L cells but not in peritoneal resident macrophages. These proteins were isolated from lysates of L cells and separated into four distinct proteins by preparative SDS‐polyacrylamide gel electrophoresis, with molecular weights of 25K, 28K, 33K, and 35K. The individual proteins formed a complex with purified mouse factor B at a molecular ratio of 1: 1 and inhibited its hemolytic activity. Proteins 25K and 28K inhibited the hemolytic activity of an activated form of factor B combined with cobra venom factor as well as that of the native form. These inhibitors did not affect the hemolytic activity of the second component of complement in mouse serum. The inhibitory activity of the 25K protein was partially inhibited by antiserum raised against it in rabbits.

UR - http://www.scopus.com/inward/record.url?scp=0021076794&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021076794&partnerID=8YFLogxK

U2 - 10.1111/j.1348-0421.1983.tb02941.x

DO - 10.1111/j.1348-0421.1983.tb02941.x

M3 - Article

C2 - 6374390

AN - SCOPUS:0021076794

VL - 27

SP - 1079

EP - 1091

JO - Microbiology and Immunology

JF - Microbiology and Immunology

SN - 0385-5600

IS - 12

ER -