Endogenous angiogenin in endothelial cells is a general requirement for cell proliferation and angiogenesis

Koji Kishimoto, Shumei Liu, Takanori Tsuji, Karen A. Olson, Guo Fu Hu

Research output: Contribution to journalArticle

216 Citations (Scopus)

Abstract

Angiogenin is an angiogenic protein that undergoes nuclear translocation in endothelial cells where it accumulates in the nucleolus and stimulates rRNA transcription, a rate-limiting step in ribosome biogenesis, protein translation, and cell growth. Here, we report that angiogenin is required for cell proliferation induced by various other angiogenic proteins including acidic and basic fibroblast growth factors (aFGF and bFGF), epidermal growth factor (EGF), and vascular endothelial growth factor (VEGF). Downregulation of angiogenin in endothelial cells by small interfering RNA (siRNA) and antisense results in a decrease in rRNA transcription, ribosome biogenesis, and cell proliferation induced by these angiogenic factors. Inhibitors of the nuclear translocation of angiogenin abolish the angiogenic activities of these factors. Stable angiogenin antisense transfection in HeLa cells reduces tumor angiogenesis in athymic mice despite the elevated expression level of bFGF and VEGF. Thus, nuclear angiogenin assumes an essential role in endothelial cell proliferation and is necessary for angiogenesis induced by other angiogenic factors. Angiogenin-stimulated rRNA transcription in endothelial cells may thus serve as a crossroad in the process of angiogenesis induced by various angiogenic factors.

Original languageEnglish
Pages (from-to)445-456
Number of pages12
JournalOncogene
Volume24
Issue number3
DOIs
Publication statusPublished - Jan 13 2005

Fingerprint

Endothelial Cells
Cell Proliferation
Angiogenesis Inducing Agents
Angiogenic Proteins
Ribosomes
Vascular Endothelial Growth Factor A
Fibroblast Growth Factor 1
Protein Biosynthesis
Fibroblast Growth Factor 2
angiogenin
HeLa Cells
Epidermal Growth Factor
Nude Mice
Small Interfering RNA
Transfection
Down-Regulation
Growth
Neoplasms

Keywords

  • Angiogenesis
  • Angiogenic factors
  • Angiogenin
  • Nuclear translocation
  • Ribosome biogenesis
  • RRNA transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Endogenous angiogenin in endothelial cells is a general requirement for cell proliferation and angiogenesis. / Kishimoto, Koji; Liu, Shumei; Tsuji, Takanori; Olson, Karen A.; Hu, Guo Fu.

In: Oncogene, Vol. 24, No. 3, 13.01.2005, p. 445-456.

Research output: Contribution to journalArticle

Kishimoto, Koji ; Liu, Shumei ; Tsuji, Takanori ; Olson, Karen A. ; Hu, Guo Fu. / Endogenous angiogenin in endothelial cells is a general requirement for cell proliferation and angiogenesis. In: Oncogene. 2005 ; Vol. 24, No. 3. pp. 445-456.
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