Endodontic Infection–induced Inflammation Resembling Osteomyelitis of the Jaws in Toll-like Receptor 2/Interleukin 10 Double-knockout Mice

Hajime Sasaki, Hisako Furusho, Daniel B. Rider, Justine M. Dobeck, Winston Patrick Kuo, Akira Fujimura, Subbiah Yoganathan, Kimito Hirai, Shuang Xu, Kei Sasaki, Philip Stashenko

Research output: Contribution to journalArticle

Abstract

Introduction: In general, mice develop chronic and nonhealing periapical lesions after endodontic infection. Surprisingly, we recently found that toll-like receptor 2 (TLR2)/interleukin 10 (IL-10) double-knockout (dKO) mice exhibited acute but resolving osteomyelitislike inflammation. In this study, we examined the kinetics of endodontic infection–induced inflammation in TLR2/IL-10 dKO mice and explored a potential mechanism of periapical wound healing mediated by the hypoxia-inducible factor 1 alpha (HIF-1α) subunit and arginase 1. Methods: TLR2/IL-10 dKO and wild-type C57BL/6J mice were subjected to endodontic infection in the mandibular first molars. Mice were sacrificed on days 0 (noninfected), 10, and 21 postinfection. The extent of bone destruction, inflammation, bone deposition, and gene expression were determined by micro–computed tomographic imaging, histology, bone polychrome labeling, and microarray analysis. In addition, the effect of blocking endogenous HIF-1α was tested in infected TLR2/IL-10 dKO mice using the specific inhibitor YC-1. Results: Infected TLR2/IL-10 dKO mice exhibited extensive bone destruction and inflammation on day 10 followed by spontaneous periapical wound healing including bone formation and resolution of inflammation by day 21 postinfection. In contrast, WT mice developed increasing chronic periapical inflammation over the 21-day observation period. Gene expression analyses and immunohistochemistry revealed that HIF-1α and arginase 1 were up-regulated in spontaneous wound healing in TLR2/IL-10 dKO mice. Blocking of HIF-1α in TLR2/IL-10 dKO mice using YC-1 resulted in significant inhibition of regenerative bone formation. Conclusions: The TLR2/IL-10 dKO mouse is a novel model resembling osteomyelitis of the jaws in which HIF-1α and arginase 1 appear to be crucial factors in spontaneous wound healing and bone repair.

Original languageEnglish
Pages (from-to)181-188
Number of pages8
JournalJournal of Endodontics
Volume45
Issue number2
DOIs
Publication statusPublished - Feb 1 2019
Externally publishedYes

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Pulpitis
Toll-Like Receptor 2
Osteomyelitis
Jaw
Knockout Mice
Interleukin-10
Hypoxia-Inducible Factor 1
Arginase
Wound Healing
Osteitis
Endodontics
Inflammation
Osteogenesis
Bone and Bones
Alpha Subunit Hypoxia-Inducible Factor 1
Gene Expression
Microarray Analysis
Infection
Inbred C57BL Mouse
Histology

Keywords

  • Arginase 1
  • hypoxia-inducible factor 1
  • mouse model
  • osteomyelitis
  • wound healing

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Endodontic Infection–induced Inflammation Resembling Osteomyelitis of the Jaws in Toll-like Receptor 2/Interleukin 10 Double-knockout Mice. / Sasaki, Hajime; Furusho, Hisako; Rider, Daniel B.; Dobeck, Justine M.; Kuo, Winston Patrick; Fujimura, Akira; Yoganathan, Subbiah; Hirai, Kimito; Xu, Shuang; Sasaki, Kei; Stashenko, Philip.

In: Journal of Endodontics, Vol. 45, No. 2, 01.02.2019, p. 181-188.

Research output: Contribution to journalArticle

Sasaki, H, Furusho, H, Rider, DB, Dobeck, JM, Kuo, WP, Fujimura, A, Yoganathan, S, Hirai, K, Xu, S, Sasaki, K & Stashenko, P 2019, 'Endodontic Infection–induced Inflammation Resembling Osteomyelitis of the Jaws in Toll-like Receptor 2/Interleukin 10 Double-knockout Mice', Journal of Endodontics, vol. 45, no. 2, pp. 181-188. https://doi.org/10.1016/j.joen.2018.10.007
Sasaki, Hajime ; Furusho, Hisako ; Rider, Daniel B. ; Dobeck, Justine M. ; Kuo, Winston Patrick ; Fujimura, Akira ; Yoganathan, Subbiah ; Hirai, Kimito ; Xu, Shuang ; Sasaki, Kei ; Stashenko, Philip. / Endodontic Infection–induced Inflammation Resembling Osteomyelitis of the Jaws in Toll-like Receptor 2/Interleukin 10 Double-knockout Mice. In: Journal of Endodontics. 2019 ; Vol. 45, No. 2. pp. 181-188.
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AU - Dobeck, Justine M.

AU - Kuo, Winston Patrick

AU - Fujimura, Akira

AU - Yoganathan, Subbiah

AU - Hirai, Kimito

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AU - Sasaki, Kei

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