Encapsulated dopamine-secreting cells transplanted into the brain: A possible therapy for Parkinson's disease

Yasuyuki Miyoshi, Isao Date, Takeshi Ono, Takashi Imaoka, Shoji Asari, Takashi Ohmoto, Hiroo Iwata

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Encapsulation of neurosecretory cells within a semipermeable membrane may possibly isolate the enclosed cells from the host immune system and allow inward diffusion of nutrients and outward diffusion of neurotransmitters. Moreover, the encapsulation procedure may prevent the tumor formation of enclosed cells, when they are derived from tumor cells. In the present study, PC12 cells, a dopaminergic cell line derived from a rat pheochromocytoma, were enclosed within in agarose/poly (styrene sulfonic acid) (agarose/PSSa) mixture and transplanted into the brains of rats (allogeneic transplantation) or guinea pigs (xenogeneic transplantation). Tyrosine hydroxylase (TH) immunoreactive PC12 cells within the microcapsules were observed in all rats and guinea pigs at least up to five weeks after transplantation. PC12 cells were round in shape and of relatively uniform small size. Although PC12 cells occasionally formed cell clusters, the formation of a tumor was not observed. The host reaction to agarose/PSSa microcapsules was minimum. The degree of glial fibrillary acidic protein (GFAP) positive astrocyte density around the microcapsules was similar to that around injection marks. There was no apparent immunological rejection around the capsules. High-performance liquid chromatography with electrochemical detection (HPLC-EC) showed basal and potassium-evoked release of dopamine from the PC12 cell-enclosed microcapsules in vitro. Although our data is preliminary, we believe that agarose/PSSa microcapsules are promising for producing semipermeable membranes that enable allo-and xenotransplantation of neurosecretory cells into the brain in the absence of systems immunosuppression. This approach is expected to be applied in Parkinson's disease in the near future.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalNeurological Surgery
Volume24
Issue number1
Publication statusPublished - 1996

Fingerprint

PC12 Cells
Capsules
Parkinson Disease
Dopamine
Styrene
Sulfonic Acids
Brain
Sepharose
Guinea Pigs
Therapeutics
Transplantation
Neoplasms
Heterologous Transplantation
Membranes
Glial Fibrillary Acidic Protein
Homologous Transplantation
Tyrosine 3-Monooxygenase
Pheochromocytoma
Astrocytes
Immunosuppression

Keywords

  • agarose/poly (styrene sulfonic acid) mixed gel
  • allograft
  • encapsulation
  • neural transplantation
  • Parkinson's disease
  • PC12 cell
  • xenograft

ASJC Scopus subject areas

  • Clinical Neurology

Cite this

Miyoshi, Y., Date, I., Ono, T., Imaoka, T., Asari, S., Ohmoto, T., & Iwata, H. (1996). Encapsulated dopamine-secreting cells transplanted into the brain: A possible therapy for Parkinson's disease. Neurological Surgery, 24(1), 35-39.

Encapsulated dopamine-secreting cells transplanted into the brain : A possible therapy for Parkinson's disease. / Miyoshi, Yasuyuki; Date, Isao; Ono, Takeshi; Imaoka, Takashi; Asari, Shoji; Ohmoto, Takashi; Iwata, Hiroo.

In: Neurological Surgery, Vol. 24, No. 1, 1996, p. 35-39.

Research output: Contribution to journalArticle

Miyoshi, Y, Date, I, Ono, T, Imaoka, T, Asari, S, Ohmoto, T & Iwata, H 1996, 'Encapsulated dopamine-secreting cells transplanted into the brain: A possible therapy for Parkinson's disease', Neurological Surgery, vol. 24, no. 1, pp. 35-39.
Miyoshi, Yasuyuki ; Date, Isao ; Ono, Takeshi ; Imaoka, Takashi ; Asari, Shoji ; Ohmoto, Takashi ; Iwata, Hiroo. / Encapsulated dopamine-secreting cells transplanted into the brain : A possible therapy for Parkinson's disease. In: Neurological Surgery. 1996 ; Vol. 24, No. 1. pp. 35-39.
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