Abstract
Pulmonary emphysema is characterized by persistent inflammation and progressive alveolar destruction. The receptor for advanced glycation end-products (RAGE) is a multiligand cell surface receptor reported to be involved in the process of acute alveolar epithelial cell injury.However, studies that address the role ofRAGE in pulmonary emphysema are inconclusive. We investigated the role of RAGE in the development of elastase-induced pulmonary inflammation and emphysema in mice. RAGE-sufficient (RAGE1/1) mice and RAGE-deficient (RAGE2/2) mice were treatedwith intratracheal elastase on Day 0. Airway inflammation, static lung compliance, lung histology, and the levels of neutrophil-related chemokine and proinflammatory cytokines in bronchoalveolar lavage fluid were determined on Days 4 and 21. Neutrophilia in bronchoalveolar lavage fluid, seen in elastase-treated RAGE1/1 mice, was reduced in elastase-treated RAGE2/2 mice on Day 4, and was associated with decreased levels of keratinocyte chemoattractant, macrophage inflammatory protein-2, and IL-1b. Static lung compliance values and emphysematous changes in the lung tissue were decreased in RAGE2/2 mice compared with RAGE1/1 mice on Day 21 after elastase treatment. Experiments using irradiated, bone marrow-chimeric mice showed that themice expressingRAGEon radioresistant structural cells, but not hematopoietic cells, developed elastase-induced neutrophilia and emphysematous change in the lung. In contrast, mice expressing RAGE on hematopoietic cells, but not radioresistant structural cells, showed reduced neutrophilia and emphysematous change in the lung. These data identify the importance of RAGE expressed on lung structural cells in the development of elastase-induced pulmonary inflammation and emphysema. Thus, RAGE represents a novel therapeutic target for preventing pulmonary emphysema.
| Original language | English |
|---|---|
| Pages (from-to) | 482-491 |
| Number of pages | 10 |
| Journal | American Journal of Respiratory Cell and Molecular Biology |
| Volume | 52 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 1 2015 |
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Keywords
- Elastase
- Emphysema
- Receptor for advanced glycation end-products
ASJC Scopus subject areas
- Cell Biology
- Pulmonary and Respiratory Medicine
- Molecular Biology
- Clinical Biochemistry
Cite this
Emphysema requires the receptor for advanced glycation end-products triggering on structural cells. / Waseda, Koichi; Miyahara, Nobuaki; Taniguchi, Akihiko; Kurimoto, Etsuko; Ikeda, Genyo; Koga, Hikari; Fujii, Utako; Yamamoto, Yasuhiko; Gelfand, Erwin W.; Yamamoto, Hiroshi; Tanimoto, Mitsune; Kanehiro, Arihiko.
In: American Journal of Respiratory Cell and Molecular Biology, Vol. 52, No. 4, 01.04.2015, p. 482-491.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Emphysema requires the receptor for advanced glycation end-products triggering on structural cells
AU - Waseda, Koichi
AU - Miyahara, Nobuaki
AU - Taniguchi, Akihiko
AU - Kurimoto, Etsuko
AU - Ikeda, Genyo
AU - Koga, Hikari
AU - Fujii, Utako
AU - Yamamoto, Yasuhiko
AU - Gelfand, Erwin W.
AU - Yamamoto, Hiroshi
AU - Tanimoto, Mitsune
AU - Kanehiro, Arihiko
PY - 2015/4/1
Y1 - 2015/4/1
N2 - Pulmonary emphysema is characterized by persistent inflammation and progressive alveolar destruction. The receptor for advanced glycation end-products (RAGE) is a multiligand cell surface receptor reported to be involved in the process of acute alveolar epithelial cell injury.However, studies that address the role ofRAGE in pulmonary emphysema are inconclusive. We investigated the role of RAGE in the development of elastase-induced pulmonary inflammation and emphysema in mice. RAGE-sufficient (RAGE1/1) mice and RAGE-deficient (RAGE2/2) mice were treatedwith intratracheal elastase on Day 0. Airway inflammation, static lung compliance, lung histology, and the levels of neutrophil-related chemokine and proinflammatory cytokines in bronchoalveolar lavage fluid were determined on Days 4 and 21. Neutrophilia in bronchoalveolar lavage fluid, seen in elastase-treated RAGE1/1 mice, was reduced in elastase-treated RAGE2/2 mice on Day 4, and was associated with decreased levels of keratinocyte chemoattractant, macrophage inflammatory protein-2, and IL-1b. Static lung compliance values and emphysematous changes in the lung tissue were decreased in RAGE2/2 mice compared with RAGE1/1 mice on Day 21 after elastase treatment. Experiments using irradiated, bone marrow-chimeric mice showed that themice expressingRAGEon radioresistant structural cells, but not hematopoietic cells, developed elastase-induced neutrophilia and emphysematous change in the lung. In contrast, mice expressing RAGE on hematopoietic cells, but not radioresistant structural cells, showed reduced neutrophilia and emphysematous change in the lung. These data identify the importance of RAGE expressed on lung structural cells in the development of elastase-induced pulmonary inflammation and emphysema. Thus, RAGE represents a novel therapeutic target for preventing pulmonary emphysema.
AB - Pulmonary emphysema is characterized by persistent inflammation and progressive alveolar destruction. The receptor for advanced glycation end-products (RAGE) is a multiligand cell surface receptor reported to be involved in the process of acute alveolar epithelial cell injury.However, studies that address the role ofRAGE in pulmonary emphysema are inconclusive. We investigated the role of RAGE in the development of elastase-induced pulmonary inflammation and emphysema in mice. RAGE-sufficient (RAGE1/1) mice and RAGE-deficient (RAGE2/2) mice were treatedwith intratracheal elastase on Day 0. Airway inflammation, static lung compliance, lung histology, and the levels of neutrophil-related chemokine and proinflammatory cytokines in bronchoalveolar lavage fluid were determined on Days 4 and 21. Neutrophilia in bronchoalveolar lavage fluid, seen in elastase-treated RAGE1/1 mice, was reduced in elastase-treated RAGE2/2 mice on Day 4, and was associated with decreased levels of keratinocyte chemoattractant, macrophage inflammatory protein-2, and IL-1b. Static lung compliance values and emphysematous changes in the lung tissue were decreased in RAGE2/2 mice compared with RAGE1/1 mice on Day 21 after elastase treatment. Experiments using irradiated, bone marrow-chimeric mice showed that themice expressingRAGEon radioresistant structural cells, but not hematopoietic cells, developed elastase-induced neutrophilia and emphysematous change in the lung. In contrast, mice expressing RAGE on hematopoietic cells, but not radioresistant structural cells, showed reduced neutrophilia and emphysematous change in the lung. These data identify the importance of RAGE expressed on lung structural cells in the development of elastase-induced pulmonary inflammation and emphysema. Thus, RAGE represents a novel therapeutic target for preventing pulmonary emphysema.
KW - Elastase
KW - Emphysema
KW - Receptor for advanced glycation end-products
UR - http://www.scopus.com/inward/record.url?scp=84929660793&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84929660793&partnerID=8YFLogxK
U2 - 10.1165/rcmb.2014-0027OC
DO - 10.1165/rcmb.2014-0027OC
M3 - Article
VL - 52
SP - 482
EP - 491
JO - American Journal of Respiratory Cell and Molecular Biology
JF - American Journal of Respiratory Cell and Molecular Biology
SN - 1044-1549
IS - 4
ER -