EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro

Yuka Nakatomi, Eichi Tsuruga, Kazuki Nakashima, Yoshihiko Sawa, Hiroyuki Ishikawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The elastic system fibers comprise oxytalan, elaunin, and elastic fibers, differing in their relative microfibril and elastin contents. Among them, human periodontal ligament (PDL) contains only oxytalan fibers (pure microfibrils). Elastin microfibril interface-located protein-1 (EMILIN-1) is localized at the interface between microfibrils and elastin. We hypothesized that EMILIN-1 may contribute to the formation of oxytalan fibers. We used a small interfering RNA (siRNA) for EMILIN-1 in PDL cell culture to examine the extracellular deposition of fibrillin-1 (the major component of microfibrils). EMILIN-1 was labeled on microfibrils positive for fibrillin-1 and was colocalized with fibrillin-1 upon immunoprecipitation assay. EMILIN-1 suppression reduced the level of fibrillin-1 deposition to 23 of the control, and this was responsible for the diminution of fibrillin-1 deposition revealed by immunofluorescence. These results suggest that EMILIN-1 may regulate the formation of oxytalan fibers and play a role in their homeostasis.

Original languageEnglish
Pages (from-to)30-35
Number of pages6
JournalConnective Tissue Research
Volume52
Issue number1
DOIs
Publication statusPublished - Feb 2011
Externally publishedYes

Fingerprint

Periodontal Ligament
Ligaments
Microfibrils
Fibers
Elastic Tissue
Elastin
Cell culture
Immunoprecipitation
Small Interfering RNA
Fluorescent Antibody Technique
In Vitro Techniques
elastin microfibril interface located protein
oxytalan
Assays
Homeostasis
Cell Culture Techniques
Fibrillin-1

Keywords

  • EMILIN
  • Fibrillin
  • Microfibrils
  • Oxytalan fiber
  • Periodontal ligament

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro. / Nakatomi, Yuka; Tsuruga, Eichi; Nakashima, Kazuki; Sawa, Yoshihiko; Ishikawa, Hiroyuki.

In: Connective Tissue Research, Vol. 52, No. 1, 02.2011, p. 30-35.

Research output: Contribution to journalArticle

Nakatomi, Yuka ; Tsuruga, Eichi ; Nakashima, Kazuki ; Sawa, Yoshihiko ; Ishikawa, Hiroyuki. / EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro. In: Connective Tissue Research. 2011 ; Vol. 52, No. 1. pp. 30-35.
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