Emergence of nuclear heparanase induces differentiation of human mammary cancer cells

Tetsuji Nobuhisa, Yoshio Naomoto, Munenori Takaoka, Yoko Tabuchi, Keizou Ookawa, Dai Kitamoto, Esra Gunduz, Mehmet Gunduz, Hitoshi Nagatsuka, Minoru Haisa, Junji Matsuoka, Motowo Nakajima, Noriaki Tanaka

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The study of epithelial differentiation touches upon many modern aspects of biology. The epithelium is in constant dialogue with the underlying mesenchyme to control stem cell activity, proliferation in transit-amplifying compartments, lineage commitment, terminal differentiation and, ultimately, cell death. There are spatially distinct compartments dedicated to each of these events. Recently we reported that heparanase is expressed in nucleus as well as in the cytoplasm and that nuclear heparanase seems to be related to cell differentiation. In this study, we investigated the role of nuclear heparanase in differentiation by transducing human mammary epithelial cancer cells with heparanase which was delivered specifically into nucleus. We observed that expression of nuclear heparanase allowed the cells to differentiate with the appearance of lipid droplets. This finding supports the idea that heparanase plays a novel role in epithelial cell differentiation apart from its known enzymatic function.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume331
Issue number1
DOIs
Publication statusPublished - May 27 2005

Fingerprint

Cells
Breast Neoplasms
Cell Differentiation
Epithelial Cells
Mesoderm
Cell death
Stem cells
heparanase
Cytoplasm
Cell Death
Stem Cells
Epithelium
Cell Proliferation
Lipids

Keywords

  • Differentiation
  • Epithelial cancer
  • Heparanase
  • MCF-7
  • Nucleus

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Nobuhisa, T., Naomoto, Y., Takaoka, M., Tabuchi, Y., Ookawa, K., Kitamoto, D., ... Tanaka, N. (2005). Emergence of nuclear heparanase induces differentiation of human mammary cancer cells. Biochemical and Biophysical Research Communications, 331(1), 175-180. https://doi.org/10.1016/j.bbrc.2005.03.129

Emergence of nuclear heparanase induces differentiation of human mammary cancer cells. / Nobuhisa, Tetsuji; Naomoto, Yoshio; Takaoka, Munenori; Tabuchi, Yoko; Ookawa, Keizou; Kitamoto, Dai; Gunduz, Esra; Gunduz, Mehmet; Nagatsuka, Hitoshi; Haisa, Minoru; Matsuoka, Junji; Nakajima, Motowo; Tanaka, Noriaki.

In: Biochemical and Biophysical Research Communications, Vol. 331, No. 1, 27.05.2005, p. 175-180.

Research output: Contribution to journalArticle

Nobuhisa, T, Naomoto, Y, Takaoka, M, Tabuchi, Y, Ookawa, K, Kitamoto, D, Gunduz, E, Gunduz, M, Nagatsuka, H, Haisa, M, Matsuoka, J, Nakajima, M & Tanaka, N 2005, 'Emergence of nuclear heparanase induces differentiation of human mammary cancer cells', Biochemical and Biophysical Research Communications, vol. 331, no. 1, pp. 175-180. https://doi.org/10.1016/j.bbrc.2005.03.129
Nobuhisa, Tetsuji ; Naomoto, Yoshio ; Takaoka, Munenori ; Tabuchi, Yoko ; Ookawa, Keizou ; Kitamoto, Dai ; Gunduz, Esra ; Gunduz, Mehmet ; Nagatsuka, Hitoshi ; Haisa, Minoru ; Matsuoka, Junji ; Nakajima, Motowo ; Tanaka, Noriaki. / Emergence of nuclear heparanase induces differentiation of human mammary cancer cells. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 331, No. 1. pp. 175-180.
@article{53caa0e2172b4532916546d4f85e3c36,
title = "Emergence of nuclear heparanase induces differentiation of human mammary cancer cells",
abstract = "The study of epithelial differentiation touches upon many modern aspects of biology. The epithelium is in constant dialogue with the underlying mesenchyme to control stem cell activity, proliferation in transit-amplifying compartments, lineage commitment, terminal differentiation and, ultimately, cell death. There are spatially distinct compartments dedicated to each of these events. Recently we reported that heparanase is expressed in nucleus as well as in the cytoplasm and that nuclear heparanase seems to be related to cell differentiation. In this study, we investigated the role of nuclear heparanase in differentiation by transducing human mammary epithelial cancer cells with heparanase which was delivered specifically into nucleus. We observed that expression of nuclear heparanase allowed the cells to differentiate with the appearance of lipid droplets. This finding supports the idea that heparanase plays a novel role in epithelial cell differentiation apart from its known enzymatic function.",
keywords = "Differentiation, Epithelial cancer, Heparanase, MCF-7, Nucleus",
author = "Tetsuji Nobuhisa and Yoshio Naomoto and Munenori Takaoka and Yoko Tabuchi and Keizou Ookawa and Dai Kitamoto and Esra Gunduz and Mehmet Gunduz and Hitoshi Nagatsuka and Minoru Haisa and Junji Matsuoka and Motowo Nakajima and Noriaki Tanaka",
year = "2005",
month = "5",
day = "27",
doi = "10.1016/j.bbrc.2005.03.129",
language = "English",
volume = "331",
pages = "175--180",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Emergence of nuclear heparanase induces differentiation of human mammary cancer cells

AU - Nobuhisa, Tetsuji

AU - Naomoto, Yoshio

AU - Takaoka, Munenori

AU - Tabuchi, Yoko

AU - Ookawa, Keizou

AU - Kitamoto, Dai

AU - Gunduz, Esra

AU - Gunduz, Mehmet

AU - Nagatsuka, Hitoshi

AU - Haisa, Minoru

AU - Matsuoka, Junji

AU - Nakajima, Motowo

AU - Tanaka, Noriaki

PY - 2005/5/27

Y1 - 2005/5/27

N2 - The study of epithelial differentiation touches upon many modern aspects of biology. The epithelium is in constant dialogue with the underlying mesenchyme to control stem cell activity, proliferation in transit-amplifying compartments, lineage commitment, terminal differentiation and, ultimately, cell death. There are spatially distinct compartments dedicated to each of these events. Recently we reported that heparanase is expressed in nucleus as well as in the cytoplasm and that nuclear heparanase seems to be related to cell differentiation. In this study, we investigated the role of nuclear heparanase in differentiation by transducing human mammary epithelial cancer cells with heparanase which was delivered specifically into nucleus. We observed that expression of nuclear heparanase allowed the cells to differentiate with the appearance of lipid droplets. This finding supports the idea that heparanase plays a novel role in epithelial cell differentiation apart from its known enzymatic function.

AB - The study of epithelial differentiation touches upon many modern aspects of biology. The epithelium is in constant dialogue with the underlying mesenchyme to control stem cell activity, proliferation in transit-amplifying compartments, lineage commitment, terminal differentiation and, ultimately, cell death. There are spatially distinct compartments dedicated to each of these events. Recently we reported that heparanase is expressed in nucleus as well as in the cytoplasm and that nuclear heparanase seems to be related to cell differentiation. In this study, we investigated the role of nuclear heparanase in differentiation by transducing human mammary epithelial cancer cells with heparanase which was delivered specifically into nucleus. We observed that expression of nuclear heparanase allowed the cells to differentiate with the appearance of lipid droplets. This finding supports the idea that heparanase plays a novel role in epithelial cell differentiation apart from its known enzymatic function.

KW - Differentiation

KW - Epithelial cancer

KW - Heparanase

KW - MCF-7

KW - Nucleus

UR - http://www.scopus.com/inward/record.url?scp=20244381142&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20244381142&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.03.129

DO - 10.1016/j.bbrc.2005.03.129

M3 - Article

C2 - 15845375

AN - SCOPUS:20244381142

VL - 331

SP - 175

EP - 180

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -